FIM-0916菌株中代谢产物的抗菌活性及稳定性考察

Studies on the antibacterial aitivity and Stability of the active component producing by Streptomyces sp. FIM-0916

目的鉴定链霉菌Streptomyces sp.FIM-0916发酵液中的抗菌活性代谢产物并探讨其化合物的稳定性。方法采用大孔吸附柱层析、常压反相色谱、高效液相色谱等方法对FIM-0916菌株发酵液进行分离纯化,通过现代波谱分析对获得抗菌活性物质0916A进行鉴定,并考察其稳定性。结果从FIM-0916菌株发酵液中分离到1个环脂肽类化合物并具有相关抑菌活性,根据理化性质研究和波谱分析,证实该化合物与Amphomycin(安福霉素)同质,该化合物在在偏碱性水溶液、低温温度条件下稳定。结论从FIM-0916发酵液中分离得到环脂肽化合物0916A,与amphomycin同质,有机溶剂、pH、温度对0916A的稳定性具有重要影响。活性研究表明该化合物对革兰氏阳性菌如枯草芽孢杆菌、耐药金黄色葡萄球菌等具有明显的抑制活性。

OBJECTIVE To identify the antibacterial compound extracted from the fermentation broth of Strep-tomyces sp.0916 and investigate its stability.METHODS The secondary metabolites were isolated by macroporous resin column chromatography ,reversion phase chromatography and high performance liquid chromatography （ HPLC）.The chemical structure of compound 0916A was identified by UV,MS,IR and NMR spectras.And its stability was studied.RESULTS The active cyclo-lipopeptide compound isolated from the fermentation broth of FIM-0916 was i-dentified as amphomycin on the basis of physicochemical property and spectral data .it is more stable in the water of pH 7.0~9.0 at lower temperature.CONCLUSION Compound 0916A,isolated from the broth of strain FIM0916, is identical with amphomycin.The organic solvent,pH,and temperature have important influence on its stability.It＆amp;nbsp;shows obvious growth inhibitory activity against the Bacillus subtilis ,MRSA and other gram-positive bacteria.