金线莲茎腐病菌的生物学特性及5种杀菌剂对其抑制作用

Biological characteristics of Fusarium oxysporum and inhibitory effects of five fungicides

采用十字交叉法和血球计数板法分别测定不同培养温度、pH和光照条件对金线莲茎腐病菌菌丝生长状况和产孢量的影响；用菌丝生长速率法测定5种杀菌剂对金线莲茎腐病菌的抑制作用，为金线莲茎腐病的防治提供参考。结果表明该病菌菌丝生长、产孢的最适温度都为28℃，最适生长的pH为6～7，且光暗交替可促进菌落的生长和产孢。在5种杀菌剂中，戊唑醇乳油的EC50为10．02mg·L-1，为多菌灵的92．50倍；其次是腈菌唑和福星乳油，EC50分别为91．23，96．68mg·L-1。戊唑醇乳油对金线莲茎腐病菌的抑制作用最强，防治效果较好。

The mycelium growth and sporulation in different temperature, pH value and light conditions were detected by using the crossing and haemocytometer method. The toxicity of five fungicides to Fusarium oxysporum was tested by mycelia growth method, so as to provide the reference for prevention and control against F. oxysporum. The optimum temperature and pH value of F. oxysporum to grow and spore were 28 ℃ and 6-7. Alternating light and darkness promoted growth and sporulation of bacterial colony. As for five fungicides, the EC50 of tebuconazole was 10. 02 mg L-1, 92. 50 times as much as carbendazim. The EC50 of myclobutanil and Fuxing was 91.23, 96. 68 mg L-1, respectively. Tebuconazole showed the most tremendous inhibitory effect and control efficiency on F. oxysporum.