LKB1/AMPK/mTOR信号转导通路在子宫内膜癌发病机制中的研究

Study on LKB1 /AMPK /mTOR signal transduction pathway in endometrial cancer

目的:探讨LKB1/AMPK/mTOR信号转导通路在子宫内膜癌发生及发展中的作用。方法:以30例正常增殖期子宫内膜组织为对照组,子宫内膜癌、子宫内膜非典型增生组织各30例为实验组,免疫组化方法检测各组子宫内膜组织中LKB1、AMPK、mTOR表达情况。结果:子宫内膜癌组LKB1和AMPK的表达均低于非典型增生组及增殖期子宫内膜组,而非典型增生组LKB1和AMPK的表达均低于增殖期子宫内膜组,差异均有统计学意义(P<0.05)。子宫内膜癌组mTOR表达高于非典型增生组及增殖期子宫内膜组(P<0.01),而非典型增生组mTOR表达高于增殖期子宫内膜组(P<0.01)。在增殖期子宫内膜、子宫内膜非典型增生及子宫内膜腺癌中,LKB1与AMPK表达呈正相关(P<0.01),LKB1、AMPK与mTOR表达呈负相关(P<0.01)。LKB1、AMPK表达与子宫内膜样腺癌的组织学分级相关,与年龄、FIGO分期无关;而mTOR表达则与子宫内膜样腺癌组织学分级及绝经状态有关,与患者年龄及手术病理分期无关。结论:证实了LKB1/AMPK/mTOR信号转导通路在子宫内膜癌发生及发展中发挥重要作用。

Objective： To explore the role of LKB1/AMPK/mTOR signal transduction pathway in occurrence and development of endometrial cancer. Methods： Thirty cases with normal endometrium during proliferative phase were selected as control group; thirty cases with endometrial cancer were selected as endometrial cancer group, and thirty cases with atypical hyperplasia of endometrium were selected as atypical hyperplasia group; immunohistochemical method was used to detect the expressions of LKB1, AMPK and mTOR in the three groups. Results： The expression levels of LKB1 and AMPK in endometrial cancer group were statistically significantly lower than those in atypical hyperplasia group and control group （ P 〈 O. 05 ）, the expression levels of LKBt and AMPK in atypical hyperplasia group were statistically significantly lower than those in control group （ P 〈 0. 05 ） , The expression level of mTOR in endometrial cancer group was statistically sig- nificantly higher than those in atypical hyperplasia group and control group （ P 〈 O. 01 ）, and the expression level of mTOR in atypical hyper- plasia group was statistically significantly higher than that in control group （P 〈 0. 01 ） . In the three groups, LKB1 expression was positively correlated with AMPK expression （ P 〈 0. O1 ） , LKB1 expression and AMPK expression were negatively correlated with mTOR expression （P 〈 0. O1 ） . LKB1 expression and AMPK expression were related to histological grade of endometrioid adenocarcinoma, which were not correlated with age and FIGO stage, mTOR expression was correlated with histological grade of endometrioid adenocarcinoma and menopausal status, which were not correlated with age and surgical pathological staging. Conclusion： The study confirms that LKB1/AMPK/mTOR sig- nal transduction pathway plays an important role in occurrence and development of endometrial cancer.