NF-κB反义寡核苷酸对博莱霉素致小鼠肺纤维化病理过程中NF-κB/IκB的影响

Effect of NF-κB antisense oligonucleotide on NF-κB/IκB in the pathological process of Bleomycin induced pulmonary fibrosis

目的探讨NF-κB反义寡核苷酸对博莱霉素致小鼠肺纤维化病理过程中NF-κB/IκB的影响。方法 C57BL/6肺纤维化模型小鼠造模前6 h,尾静脉注射NF-κB反义寡核苷酸,取肺组织,模型组及对照组分别行原代培养。肺组织及培养细胞进行NF-κB、IκB-α蛋白的免疫组织化学染色及细胞化学染色,并行图像分析,以平均光密度(MOD值)表示各指标的表达量。结果在肺组织及培养细胞中,模型组NF-κB、IκB-α蛋白表达量均较对照组增加(P<0.05),干预组各项指标的表达量均较模型组减少(P<0.05)。NF-κB蛋白与IκB-α蛋白表达呈正相关。结论 NF-κB反义寡核苷酸可以抑制博莱霉素致小鼠肺纤维化病理过程中NF-κB/IκB的表达,从而起到治疗肺纤维化的作用。

[Objective] To investigate the effect of NF-KB antisense oligonucleotide on NF-KB/IKB in the pathological process of Bleomycin-induced pulmonary fibrosis. [Methods] C57BL/6 modeling mice were intra- venous injected by NF-KB antisense oligonucleotides 6 hours before mice model of pulmonary fibrosis estab- lished, the lung tissue was take out and then primarily cultured. Model group and control group were primari- ly cultured respectively at the same time. NF-KB and IKB--oL protein expression of lung tissue and cultured cells were detected by immunohistochemistry and immunocytochemistry staining. After image analysis, the ex- pression levels of each index were expressed by the mean optical density value （MOD value）. [Results] The expression of NF-KB protein and IKB-ct protein of lung tissue and cultured cells in the model group were higher than those in the control group （P〈0.05）. The expression of the all indicators in the intervention group were lower than those in the model group （P〈0.05）. NF-KB protein expression was positively correlated with IKB-a protein expression respectively. [Conclusion] NF-KB antisense oligonucleotides can inhibit the expres- sion of NF-KB/IKB in the pathological process of Bleomycin-induced pulmonary fibrosis, therefore, play an important role in the treatment of pulmonarv fibrosis.