药物氟哌酸分子模板聚合物的分子识别特性

Characteristics of Molecular Recognition of Norfloxacin Template Polymer

采用分子印迹技术合成了对药物氟哌酸有高度选择性的模板聚合物。通过 Ｓｃａｔｃｈａｒｄ分析研究了模板聚合物的选择结合特性。结果表明，以甲基丙烯酸为功能单体的模 板聚合物通过离子作用和氢键作用可以形成两类结合位点。用多点结合模型计算两类不同 结合位点的离解常数分别为Ｋｄ１＝２．９×１０－５ｍｏｌ／Ｌ和Ｋ＝３．２×１０－３ｍｏｌ／Ｌ。结合底物的实 验表明，该聚合物对氟哌酸呈现出高的选择性，有明显的开发应用价值。

A molecular template synthetic polymer highly selective for norfloxacinum was prepared by a molecular imprinting technique. The selective binding characteristics of the template polymer was evaluated by Scatchard analysis. The multiple-sites binding model was used to calculate the maximum number of binding sites, and the dissociation constant. The results showed that the template polymer using methacrylic acid (MAA) as functional monomer could form two kinds of binding sites. The dissociation constants were estimated to be K-d1 = 2.9 x 10(-5) mol/L and K-d2 = 3.2 x 10(-3) mol/L. The selective binding experiment for substrates indicated that the polymer gave much higher affinity and selectivity for norfloxacin than for acid pipemidic and cefalexin. It is possible to be a good adsorption and binding material in the selective enrichment and determination of trace norfloxacin in complex biosamples.