混合线性分析-分光光度法同时测定微量铁和铝

Simultaneous Spectrophotometric Determination of Trace Iron(Ⅲ)and Aluminum (Ⅲ)with Hybrid Linear Analysis

研究将混合线性分析（ｈｙｂｒｉｄ ｌｉｎｅａｒ ａｎａｌｙｓｉｓ，ＨＬＡ）用于光谱分辨同时光度测定Ｆｅ３＋和Ａｌ３＋。在碱性条件下，Ｆｅ３＋和Ａｌ３＋与水杨基荧光团生成光谱相互重叠的有色络合物，其浓度在０～３２０ ｇ／Ｌ Ｆｅ３＋和０～１６０ｇ／Ｌ Ａｌ３＋范围内与吸光度呈良好线性关系。在５５０ｎｍ～６３０ ｎｍ内取４１个波长测量７组标准混合溶液的吸光度作为校正集，用最小二乘法求得的系数矢量作为各组分的纯光谱。以ＨＬＡ法进行数据处理，在Ｆｅ３＋：Ａｌ３＋浓度为１０：１～１：８范围内测定回收率为９３．３％～１０７．５％。将本法与偏最小二乘法（ＰＬＳ）法进行了比较，本法获得了较好的准确度和精密度。

Hybrid linear analysis (HLA) was applied to resolution of overlapping spectra of Fe3+-salicylfluorone and A13+ -salicyifluorone complexes and simultaneous spectrophotometric determination of Fe3+ and Al3+. The absorbance matrix of 7 standard mixtures at 41 measuring points ranged form the wavelength of 550 nm to 630 nm was used for calibration.To avoid the effect of interaction between the two components on the determination, the column vector of K matrix obtained form the standard mixtures with least squares was used as the pure spectrum of component.The recoveries of the two elements for the analysis of the analysis of the synthetic samples were 93.3% ~ 107.5% in the range of the concentration ratio of Fe3+:Al3+=10:1 to 1:8. Comparing with the partial least squares (PLS) model,the HLA method was simple,accuracy and precise.