用荧光法研究补骨脂素与异补骨脂素的药代动力学

Study on the of Pharmacokinetics of Psoralen and Isopsoralen by Fluorometry

补骨脂素和异补骨脂素是补骨脂的主要有效成分。为了研究补骨脂素和异补骨脂素的药物代谢动力学，本文采用胶束荧光法对补骨脂素和异补骨脂素在兔体内的血药浓度进行２４ｈ监测，求出系列药动学参数。结果表明：补骨脂素的Ｋａ为３．２２５１ｈ－１，ｔ１／２（α）为 ０．１８４９ｈ，ｔ１／２（β）为１０．７０６５ｈ，Ｔ为０．８２００ｈ，ＡＵＣ为２１．６１０４ｍｇ·ｈ／Ｌ；异补骨脂素的Ｋａ为４．４３２９ｈ－１，ｔ１／２（Ｋａ）为０．１５６４ｈ，ｔ１／２（Ｋｅ）为２．１７７７ｈ，Ｔ为１．０９５５ｈ，ＡＵＣ为７．２４１８ｍｇ·ｈ／Ｌ。

Psoralen and isopsoralen are the main effective content in fructus psoraleae.In order to study the relatiog between pharmacodynmice and pharmacokinetics,micellar fluorometry was used to monitor berberine blood-drug conentaion in rabbit for 24 hours, and a series of pharmacodinetics were obtained. Those are Ka = 3.225h-1, t1/2(a) = 0. 1849h, t1/2 (β) = 10.7065h, T = 0. 8200h,AUC = 21 .6l04 x 10-6 mg·h/L for psoralen, and Ka = 4.4329h-1, t1/2 (Ka) = 0.156h, t1/2 (Ka ) = 2.1777h, T = 1. 0955h, AUC = 7. 2418 x 10-6 g.h/ml, for isopsoralen.