丝素膜固定化丝网印刷酶电极

Screen-Printing Enzyme Electrode-Immobilizing the Enzyme with Silk Fibroin

采用丝网印刷技术制作的一次性薄层碳电极具有良好的分析性能。经化学修饰二茂铁后，以丝素为固定化载体固定含有多酚氧化酶的蘑菇萃取蛋白，所得酸电极对其底物邻苯二酚和多巴胺有良好响应，线性范围为２．０×１０－８～２．０×１０－２，ｍｏｌ／Ｌ，在３０ｓ内即可达到９５％稳态电流。该电极的ＲＳＤ为２．７％，且可保持１０天以上的工作寿命。该酶电极在低温湿润环境中可保存较长时间，因此有较好的商品化前景。

This paper reports the technique to develop a disposable screen-printing base carbon electrode for the utilization in the study of enzyme electrode. The silk fibroin was applied to immobilize the mushroom extracted protein, which contained abundant polyphenol oxidase, on the surface of base electrode when it was chemically moldified by ferrocene. The voltammetric current of this enzyme electrode responded to the concentration of substrate such as catechol or dopamine. The linear range is from 2.0 x 10(-8) mol/L to 2.0 x 10(-2) mol/L, it reaches to a 95% steady value of current within less than 30 seconds, and the HSD equals to 2.7%. The service life of this enzyme electrode is at least 10 days and it will be preserved for a longer period at a humid and cool condition. All of these performances of the enzyme electrode make clear that its commercial development would be very possible.