“复瓣玫瑰红”组织培养快繁体系的建立

Establishment of Rapid in-vitro Propagation System for Rosa multiflora var. ‘multipetal Rose-red '

以“复瓣玫瑰红”茎段为外植体，进行腋芽取材时期、腋芽节径、培养基中无机盐离子浓度、不同激素配比和组培苗驯化移栽试验。结果表明：“复瓣玫瑰红”蔷薇取材的最佳时期是5月和11月，所取材料是当年生枝条的中间部分，采用1／2MS为基本培养基并添加0．1mg／LNAA＋1．0mg／L6-BA的激素组合腋芽萌发效果最好，其萌发率最高可达100％；腋芽分化的最佳培养基为：1／2MS＋0．2mg／LNAA＋2．0mg／L6．BA，增殖系数达到8-3；最佳生根培养基为1／2MS＋0．1mg／LNAA＋0．2mg／L6-BA组合，生根率可达97．1％；组培苗移栽时，以纯蛭石为基质，先将生根瓶苗移至炼苗室进行9～13d的移栽前锻炼，并保持雾状喷水，移栽成活率可达80．0％。

Experiments were conducted on effect of sampling time, explants types, different cultures and different hormones ratio on rapid in-vitro propagation of Rosa multiflora vat. ＇raultiprtai Rose-red＇ using the stem segment as the explant. The results showed that the best sampling time was in May and November, the best stem segment was the semi-lignified branches in the middle of the shoots. The germination rate of bud topped to 100% when the medium was I/2MS supplemented with 0.1mg/L NAA and 1.0mg/L 6=BA. The optimal medium for multiplication of adventitious buds was I/2MS supplemented with 0.2mg/L NAA and 2.0mg/L 6-BA, and the multiplication coefficient was up to 8.3. The best medium for rooting was I/2MS supplemented with 0.1mg/L NAA and 0.2mg/L 6-BA, and the rooting rate was up to 97.1%. The conservation rate after transplanting reached up to 80.0% using the pure vermiculite as substrate aRer 9-13 days acclimatization, and keeping mist spray.