摘要
以海滨木巴戟(Morinda citrifolia)的嫩芽为外植体进行组培研究,以1/2MS+蔗糖30 g/L+琼脂6 g/L为基本培养基,以TDZ、BA、IBA三种激素进行不定芽的诱导分化实验,以IBA、NAA进行生根实验。结果表明,各因素对外植体分化的影响皆不显著(p<0.05),IBA抑制外植体分化;在只添加BA的培养基中,随着BA浓度的升高,外植体玻璃化的现象有增加的趋势;生根实验结果表明,NAA和IBA诱导生根效果相差不大(p<0.05),但是IBA在外植体基部诱导出更多的愈伤组织。
The axillary buds ofMorinda citrifolia were used as the explants to establish the micro-propagation system. The orthogonal experiment of bud differentiation was carried out by TDZ, BA and IBA. The results showed that three hormones had no significant（p 〈 0.05） effect on bud differentiation, but IBA inhibited the differentiation. The number of vitrification shoots increased with the concentration of BA when the medium was only added BA. Experiment on rooting induction demonstrated that the influences of NAA and IBA had no obvious difference （p 〈 0.05）. However, more callus were induced from the base of explant in the medium with IBA.
出处
《浙江林业科技》
北大核心
2013年第5期81-84,共4页
Journal of Zhejiang Forestry Science and Technology
基金
浙江省花卉新品种选育重大科技专项重点项目(2012C12909-5)
浙江省重大科技专项重点农业项目(2013F10020)
关键词
海滨木巴戟
组培
分化
生根
Morinda citrifolia
tissue culture
differentiation
rooting
