摘要
应用敏感的单克隆抗体捕获夹心法酶联免疫吸附试验,检测202例各型HBV感染者血清IgA抗-HBc.在1∶1000稀释度,重肝、肝硬化、慢活肝、急乙肝、肝癌、慢迁肝和无症状HBsAg携带者IgA抗-HBc阳性率分别为100%、89.47%、89.19%、87.5%、85%、77.5%、和67.5%在1∶5000稀释度,急乙肝的S/N值(X±SD)是7.59±3.40,显著高于慢活肝(5.62±3.93)、慢迁肝(5.05±3.19)和无症状HBsAg携带者(1.99±2.74)(P<0.05).结果提示IgA抗-HBc与肝损害程度密切相关;对急乙肝的诊断和预后判断有一定意义;多数无症状HBsAg携带者有肝损害.
IgA anti- HBc were determined by an antibody captured Sandwichtype enzyme linked immunosorbant assay with monoclonal antibody in the sera of 202 cases of various types of HBV infected patients.At 1∶1000 dilution, the positive rates of IgA anti-HBc in patients with severe hepatitis, cirrhosis, chronic active hepatitis, acute hepatitis, hepatocelluar carcinoma, chronic persistent hepatitis, and asymptomatic carriers were 100%, 89.47%, 89.1,%87.5%,85.0%, 77.5%, and 67.5%, respectively.At 1∶5000 dilution, the mean SD titer of the antibody in acute hepatitis (7.59±140) was significantly higher than that in CAH (5.62±3.93), CPH (5.05±3.19) and ASC (1.99±274) (P<0.05).The activity of IgA anti-HBc in acute hepatitis was related to the icterus index. Our results suggest that IgA anti-HBc may reflect hepatic injury and may be useful in the diagnosis and prognosis of acute hepatitis R Most of the ASC patients may have hepatic injury.
