丹参酮ⅡA磺酸钠对转换生长因子-β1诱导的心房成纤维细胞分化的影响

Effects of Sodium Tanshinone ⅡA Sulfonate on TGF-β1-Induced Atrial Fibroblasts Differentiation

目的观察丹参酮ⅡA磺酸钠（sodium tanshinone ⅡA sulfonate，STS）对转换生长因子-β1（transforminggrowth factor-β1，TGF—B1）诱导的心房成纤维细胞分化的影响及其机制。方法体外培养1～2d龄乳鼠心房成纤维细胞，酶联免疫吸附测定法检测培养上清液中Ⅰ／Ⅲ型胶原含量，四甲基偶氮唑蓝法检测细胞活力，免疫荧光法及蛋白质印迹法测定α-平滑肌肌动蛋白表达，同时蛋白质印迹法检测细胞外调节蛋白激酶1／2磷酸化。结果转化生长因子-β1能显著提高心房成纤维细胞培养上清液中Ⅰ／Ⅲ型胶原含量，提高细胞增殖活力，同时上调α-平滑肌肌动蛋白表达及细胞外调节蛋白激酶1／2磷酸化。以上各指标能被丹参酮ⅡA磺酸钠（3，10，30μmol．L^-1）逆转。结论丹参酮ⅡA磺酸钠可以抑制转化生长因子-β1诱导的心房成纤维细胞分化，机制可能与下调细胞外调节蛋白激酶1／2活化有关。

OBJECTIVE To observe the effects and mechanisms of sodium tanshinone Ⅱ A sulfonate （STS） on transforming growth factor-β1 （TGF-β1）-induced atrial fibroblasts differentiation. METHODS In the culture of neonatal rat atrial fibro-blasts, type Ⅰ/Ⅲ collagen in the cell culture supernatant were measured by enzyme-linked immunosorbent assay. The prolifera-tion activity was measured by methyhhiazolyl tetrazolium（MTT） assay. Atrial fibroblasts differentiation was determined by meas-uring the expression of α-SMA（ α-smooth muscle actin） using Western blot and immunofluorescence. Extracellular signal-regula-ted kinase 1/2（ERK1/2） activation was measured by Western blot. RESULTS STS can decrease TGF-β1-induced elevations of type Ⅰ/Ⅲ collagen and proliferation activity; inhibit the expression of α-SMA and ERK1/2 activation. CONCLUSION STS can inhibit TGF-β1-induced atrial fibroblasts differentiation, the mechanism may be associated with depression of ERK1/2 signaling pathway.