摘要
目的建立高效液相色谱(HPLC)法同时测定中药制剂烧伤膏中5个蒽醌类化合物(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)的含量。方法以5个蒽醌类成分为指标,选择DiainonsilC18(4.6mm×250mm,5μm)色谱柱,使用甲醇-乙腈旬.1%磷酸(23:42:35)为流动相进行洗脱。结果5个蒽醌类成分分别在0.0055~0.1761μg(芦荟大黄素r=0.9999)、0.0101~0.3237μg(大黄酸r=0.9999)、0.0166~0.5223μg(大黄素r=0.9999)、0.0216~0.6906μg(大黄酚r=0.9999)和0.0303~0.9688μg(大黄素甲醚r=0.9999)内,与峰面积呈良好的线性关系;平均回收率分别为97.6%、96.6%、98.8%、99.2%、97.5%;RSD(n=6)分别为2.0%、1.8%、2.9%、1.3%、1.2%。结论该方法简便、可靠、重复性好,适用于烧伤膏中大黄的质量控制。
OBJECTIVE To establish a method for determining the contents of aloe-emodin, rhein, emodin, chrysophanol and physcion in the burn ointment. METHODS Diamonsil C18 column (4. 6 mm ×250 mm,5 μm) was adopted, with methanol-acetoni-trile-0. 1% phosphoric acid (23: 42: 35) as the mobile phase. The detection wavelength was set at 254 nm, the flow rate was 1 mL · min^-1 , and the column temperature was 30 ℃. RESULTS The liner rangers of aloe-emodin, rhein, emodin, chrysophanol and phy-scion were within 0. 005 5 - 0. 176 1 μg ( r = 0. 999 9 ), 0. 010 1 - 0. 323 7 μg ( r = 0. 999 9), 0. 016 6 - 0. 522 3 μg ( r = 0. 999 9 ), 0. 021 6-0. 690 6 μg (r =0. 999 9) and 0. 030 3-0. 968 8 μg (r =0. 999 9), respectively. The average recoveries were 97.6% (RSD=2.0%),96.6% (RSD=1.8%), 98.8% (RSD=2.9%),99.2% (RSD=1.3%), and 97.5% (RSD=1.2%), respec-tively (n = 6). CONCLUSION This nlethod is specific, accurate, reproducible, and can be used for the determination of aloe-emo-din, rhein, emodin, chrysophanol and physcion in the burn ointment.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2014年第8期688-692,共5页
Chinese Pharmaceutical Journal
基金
国家自然科学基金面上资助项目(81173632)
安徽省卫生厅中医药科研资助项目(2012zy52)
2013年安徽省省级中医药发展专项基金资助项目
安徽省高等教育振兴计划人才项目省学术带头人培养资助项目(2013)