高效液相色谱法同时测定中药制剂烧伤膏中芦荟大黄素等5个蒽醌类化合物含量

Simultaneous Determination of Five Anthraquinone Compounds in Burn Ointment by HPLC

目的建立高效液相色谱（HPLC）法同时测定中药制剂烧伤膏中5个蒽醌类化合物（芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）的含量。方法以5个蒽醌类成分为指标，选择DiainonsilC18（4．6mm×250mm，5μm）色谱柱，使用甲醇-乙腈旬．1％磷酸（23：42：35）为流动相进行洗脱。结果5个蒽醌类成分分别在0．0055～0．1761μg（芦荟大黄素r=0．9999）、0．0101～0．3237μg（大黄酸r=0．9999）、0．0166～0．5223μg（大黄素r=0．9999）、0．0216～0．6906μg（大黄酚r=0．9999）和0．0303～0．9688μg（大黄素甲醚r=0．9999）内，与峰面积呈良好的线性关系；平均回收率分别为97．6％、96．6％、98．8％、99．2％、97．5％；RSD（n=6）分别为2．0％、1．8％、2．9％、1．3％、1．2％。结论该方法简便、可靠、重复性好，适用于烧伤膏中大黄的质量控制。

OBJECTIVE To establish a method for determining the contents of aloe-emodin, rhein, emodin, chrysophanol and physcion in the burn ointment. METHODS Diamonsil C18 column （4. 6 mm ×250 mm,5 μm） was adopted, with methanol-acetoni-trile-0. 1% phosphoric acid （23： 42： 35） as the mobile phase. The detection wavelength was set at 254 nm, the flow rate was 1 mL · min^-1 , and the column temperature was 30 ℃. RESULTS The liner rangers of aloe-emodin, rhein, emodin, chrysophanol and phy-scion were within 0. 005 5 - 0. 176 1 μg （ r = 0. 999 9 ）, 0. 010 1 - 0. 323 7 μg （ r = 0. 999 9）, 0. 016 6 - 0. 522 3 μg （ r = 0. 999 9 ）, 0. 021 6-0. 690 6 μg （r =0. 999 9） and 0. 030 3-0. 968 8 μg （r =0. 999 9）, respectively. The average recoveries were 97.6% （RSD=2.0%）,96.6% （RSD=1.8%）, 98.8% （RSD=2.9%）,99.2% （RSD=1.3%）, and 97.5% （RSD=1.2%）, respec-tively （n = 6）. CONCLUSION This nlethod is specific, accurate, reproducible, and can be used for the determination of aloe-emo-din, rhein, emodin, chrysophanol and physcion in the burn ointment.