带电溶质在电色谱中柱内富集方法的研究——基本理论模型和实验验证

Study on On-column Concentration of Ionic Sample in Capillary Electrochromatography——Model Development and Investigation

在电色谱 (CEC)中引入场增强进样技术 ,并与HPLC柱内富集技术结合以使带电溶质得到更好的富集效果 .理论研究表明 ,采用较高电阻率的样品溶剂 ,及匹配适当的有机调节剂强度有利于带电溶质在CEC中的柱内富集 .以碱性药物普罗帕酮为实验样品 ,采用水 ( φ =40 % ) -乙腈 ( φ =60 % )为样品溶剂 ,水 ( φ =15 % ) -乙腈 ( φ =85 % ) - Tris ( 2mmol/L) -TEA( 0 .6mmol/L ,pH 7.60 )为运行缓冲溶液时 ,达到了 17,0 0 0倍以上的富集效果 .从理论和实验研究的角度说明了CZE与HPLC机制结合的CEC进样方法用于样品柱内富集的可行性 。

Concentration process of ionic sample in capilary electrochromatography (CEC) could be carried out through association of separation mechanism of HPLC and capillary electrophoresis.A model was developed to study the concentration process in CEC. The results show that high resistivity sample,with high organic modifier concentration,could result in excelent concentration performance. A basic drug propatene was chosen at testing sample, which provide over 17,000 times of concentration. This proves the feasibility of the association mechanism of HPLC and capillary electrophoresis.Effects of operation conditions on concentration performance were also studied.