摘要
目的:建立检查药用蜂蜜真伪的色谱检测方法,为中国药典蜂蜜标准的修订提供依据。方法:试样经活性炭一硅藻土柱处理,采用薄层色谱法和高效液相色谱-蒸发光散射检测器检测法检测,色谱分离选用Prevail Carbohydrate ES5u色谱柱(4.6mm×250mm,5μm),流动相为乙腈一水,梯度洗脱(0—40min,75%A→55%A,25%B→45%B),检测寡糖成分的检出。结果:以麦芽糖系列寡糖对照,检测结果显示,天然蜂蜜不含有五糖以上的寡糖;而果葡糖浆、麦芽糖浆及其蜂蜜的掺伪品均可检出大于麦芽五糖的寡糖。结论:可通过检测寡糖的存在与否鉴别蜂蜜的真伪。
Objective: To establish methods for the determination of the "authenticity" of honey,and provide basis for the standard revision of Chinese Pharmacopoeia. Methods: The sample was processed by a column packed with activated carbon and kieselguhr, separated by TLC and HPLC - ELSD methods. The analysis was performed on Pre- vail Carbohydrate ES 5u chromatographic column(4.6mm×250mm,5μm)with a gradient elution program using mobile phase of aeetonitrile and water (0 - 40 min,75 % A→55 % A,25 % B→45 % B). The presence of oligosaccha- tides, molecular weight larger than mahopentaose, was detected in the sample. Results: No oligosacchatide was de- tected in the batches of natural honey samples, which was found in high fructose corn syrup, maltose syrup, and the honey adulteration products. Conclusion: Oligosaccharide could be adopted as a marker for the authenticity of natu- ral honey
出处
《药物分析杂志》
CAS
CSCD
北大核心
2014年第4期737-741,共5页
Chinese Journal of Pharmaceutical Analysis