薄层色谱和高效液相色谱法鉴别真伪蜂蜜的方法研究

Method study for the determination of adulteration honey by TLC and HPLC

目的：建立检查药用蜂蜜真伪的色谱检测方法，为中国药典蜂蜜标准的修订提供依据。方法：试样经活性炭一硅藻土柱处理，采用薄层色谱法和高效液相色谱-蒸发光散射检测器检测法检测，色谱分离选用Prevail Carbohydrate ES5u色谱柱（4．6mm×250mm，5μm），流动相为乙腈一水，梯度洗脱（0—40min，75％A→55%A，25％B→45％B），检测寡糖成分的检出。结果：以麦芽糖系列寡糖对照，检测结果显示，天然蜂蜜不含有五糖以上的寡糖；而果葡糖浆、麦芽糖浆及其蜂蜜的掺伪品均可检出大于麦芽五糖的寡糖。结论：可通过检测寡糖的存在与否鉴别蜂蜜的真伪。

Objective： To establish methods for the determination of the ＂authenticity＂ of honey,and provide basis for the standard revision of Chinese Pharmacopoeia. Methods： The sample was processed by a column packed with activated carbon and kieselguhr, separated by TLC and HPLC - ELSD methods. The analysis was performed on Pre- vail Carbohydrate ES 5u chromatographic column（4.6mm×250mm,5μm）with a gradient elution program using mobile phase of aeetonitrile and water （0 - 40 min,75 % A→55 % A,25 % B→45 % B）. The presence of oligosaccha- tides, molecular weight larger than mahopentaose, was detected in the sample. Results： No oligosacchatide was de- tected in the batches of natural honey samples, which was found in high fructose corn syrup, maltose syrup, and the honey adulteration products. Conclusion： Oligosaccharide could be adopted as a marker for the authenticity of natu- ral honey