外源一氧化氮对盐胁迫下白皮黄瓜种子萌发的影响

Effect of Nitric Oxide on Seed Germination of the White Cucumber Under Salt Stress

以白皮黄瓜品种"白叶三"新鲜种子为试材,采用培养皿发芽法,研究了不同浓度的NO供体硝普钠(SNP)溶液对100mmol/L NaC1胁迫下黄瓜种子萌发过程中吸水率、发芽率、相对电导率和α?淀粉酶活性的影响。结果表明:适当浓度的SNP溶液能明显提高盐胁迫下白皮黄瓜种子吸水率和发芽率,缓解盐胁迫对种子细胞膜修复的抑制作用,抑制盐胁迫下种子α?淀粉酶活性的降低;其中,以0.10mmol/L的SNP溶液减缓盐害效果较好。

Taking ＇Baiyesan＇ fresh seeds of white cucumber as material,by using culture dishes sprout method,the water absorption rate,germination rate, relative conductivity and a-amylase activity of white cucumber treated by SNP, an exogenous nitric oxide donor, at the different concentration under 100 mmol/L NaCl stress were studied. The results showed that a certain concentration of SNP treatment could alleviate the damage of NaC1 to the seeds, and increase the water absorption rate and germination rate significantly. At the same time, SNP treatment also could alleviate the inhibitory of membrane restoration and the decrease of a-amylase activity under salt stress, and the concentration of 0. 10 mmol/L SNP was the best.