CTP-3和微波处理对食用菌栽培基质三种常见杂菌的灭菌效果研究

Disinfection Test on Three Kinds of Bacterium in the Edible Fungi Treated With CTP-3 and Microwave

以食用菌栽培基质为试材,采用新型环保消毒剂CTP-3和微波方法,研究了100%、50%、25%、10%、5%、2.5%6种CTP-3浓度和30、60、90、120、150、180、210、240、270、300s10个微波作用时间对食用菌常用栽培基质中大肠杆菌、枯草芽孢杆菌、木霉菌的灭菌效果,并选取其中最难以清除的菌体使用微波和消毒剂共同作用,观察杀菌效果。结果表明:消毒剂作用时,枯草芽孢杆菌在CTP-3 25%浓度下还有生长;大肠杆菌和木霉菌在消毒剂浓度为10%之后才有生长;而微波作用菌液时,大肠杆菌在微波240s后全部杀完,木霉菌在微波210s后全部杀完,而枯草芽孢杆菌在270s才全部杀灭,所以枯草芽孢杆菌是最难彻底清除的细菌;当消毒剂稀释10倍,并微波处理180s共同作用后可以完全杀菌。

Taking culture material of edible mushroom as material,with new environmental protection disinfectant CTP-3： the disinfection effect of 100 %, 50 %,25 %, 10 %, 5 %, 2. 5 % six concentration gradient,and 30,60,90,120,150,180,210, 240,270,300 s microwave respectively on most common miscellaneous bacteria （escherichia coli, bacillus subtilis, wood mould） in mushroom growing substrate were studied. One of the most difficult to remove of the bacteria use microwave and disinfectam work together. Then its sterilization effect was observated. The results showed that when disinfection function,bacillus subtilis had growth in 25% diluted disinfectant, escherichia coli and wooden mold had growth in 10% diluted of disinfectant. And when microwave function,escherichia was killed all after 240 s microwave, wooden mold was dead after 210 s microwave, but bacillus subtilis was killed after 270 s microwave, so bacillus subtilis was the most difficult competitor to kill completely;when disinfectant diluted 10% role,and microwave processing 180 s were the best combination to kill all miscellaneous bacteria.