辣根过氧化氢酶电极的研究

Determination of Hydroquinone with HRP Modified Electrode

以辣根过氧化氢酶修饰电极采用循环伏安法对环境污染物对对苯二酚进行了测定 ,对测定条件进行了探索 ,该电极在 p H7,H2 O2 浓度为 1 0μmol· L-1时对苯二酚具有良好的响应 ,其线性范围为 2 .0× 1 0 -5～ 2 .8× 1 0 -3mol·L-1,检测下限为 1 .0× 1 0 -6mol· L-1。电极的峰电流与扫描速度的平方根成正比 ,电极上的传质过程受扩散控制 ,对酶催化反应的动力学机制进行了探讨。

Horseradish peroxidase (HRP) modified electrode was successfully used to determine hydroquinone by cyclic voltammetry. The optimum determination condition was investigated with pH7 and 10 μmol·L -1 H 2O 2. The linear relationship is held from 2.0×10 5～2 8×10 -3 mol·L -1 of hydroquinone with detection limit of 1.0×10 -6 mol·L -1 . The electrode has high response speed, good reproducibility and stability. The peak current is proportional to square root of scan rate, which indicated that the procedure is controlled by diffusion. The dynamic mechanism of the enzyme catalyzed reaction was discussed.