摘要
将抗人甲胎蛋白单克隆抗体(AFP McAb)与丝裂霉素(MMC)交联制成免疫联结物,与产AFP的人肝癌细胞株Q_3及不产AFP的K_(562)细胞株作用,然后用羊抗鼠IgG荧光抗体进行定位染色,结果显示:Q_3细胞染色呈强阳性,且定位于细胞浆区域,而K_(562)细胞染色为阴性,这表明以抗体作为抗癌药的载体具有选择定位作用,为抗体导向化疗提供了细胞学上的证据。
After the conjungant of MMC and rat McAb. against human AFP was incubatedwith human liver cancer cell line Q_3 (a AFp secreting cell line) and cell line K_(562) (a non AFPsecreting cell line), sheep fluorescent antibody specific for rat IgG was added to make aimmunofluorescent staining. The results showed that the Q_3 cells, were strongly positive to thestaining reaction and the fluorochromes were localized in the cytoplasm, but the K_(562) cellswere negative to the staining reaction. This experiment elucidates that an antibody used as adrug carrier has the ability of selective localization and provides some cytologic evidences sup-porting antibody targeting chemotherapy.
关键词
肝肿瘤
癌细胞
免疫联结物
immunoconjugant
liver cancer cell
fluorescent antibody staining
