HPLC-ICP-MS法测定农业废水中有机砷与无机砷的方法研究

Analysis of Organic and Inorganic Arsenic in Waste Water of Agricultural Environment by High Performance Liquid Chromatography and Inductively Coupled Plasma Mass Spectrometry

建立了应用高效液相色谱（HPLC）和电感耦合等离子质谱（ICP—Ms）联用技术测定农业水环境样品中三价砷（Aslll）、一甲基砷（MMA）、二甲基砷（DMA）、五价砷（AsV）4种砷形态的分析方法。试验表明，4种砷形态的线性范围宽（1-300μg·L-1），相关系数（r）均大于0．9990，方法检出限低（0．7～0．98μg·L-1），精密度好，重复测定7次结果的RSD均小于5％。通过计算加标回收率验证方法的准确性，加标回收率为94％～112％。实际样品的测定结果显示，农田废水中砷的主要存在形态为As（V），其次为As（Ⅲ）。

A high performance liquid chromatography and inductively coupled plasma mass spectrometric method was established for analyz- ing 4 arsenic speciations in water of agricultural environment, including As （ Ⅲ ）, MMA, DMA and As （ V ）. The results showed that the method had a wide linear range（ 1 - 300 μg·L-1） and the linear coefficients were more than 0.999 O. The detection limits were very low （0.7-0.9μg·L-1）. All of the relative standard deviations （RSD） were less than 5%. The recoveries were between 94% and 112%. The analysis results showed that As（ V ） and As（ 11I ） were the main arsenic speciations existed in the waste water of agricultural environment. Keywords： waste water in agricultural environment; organic arsenic; inorganic arsenic; high performance liquid chromatography-inductively coupled plasma mass spectrometric