宫颈上皮内瘤样变患者治疗后HPV感染方式的分析研究

Study on the patterns of HPV infection after treatment of CIN

目的探讨HPV的感染方式对CIN治疗后患者HPV转阴的影响。方法采用核酸分子快速导流杂交基因芯片技术检测经病理确诊的196例CIN患者治疗前及治疗后3、6、12个月HPV的感染亚型，同期检测205例正常人的HPV感染亚型。计算治疗前以及治疗不同时间后HPV不同感染方式的感染率、基因构成，并采用SPSS18．0统计软件进行统计学分析，采用X2检验比较组间的差异，研究不同感染方式对HPV转阴的影响。结果①CINI、Ⅱ、Ⅲ级患者HPV感染率分别为65．71％（46／70）、88．23％（60／68）和91．37％（53／58），均显著高于正常人群（P〈0．05）并随着CIN级别的升高而增加；②正常对照组HPV感染方式中单一高危感染所占比例最大，为6．83％（14／205），其次是混合高危感染所占比例3．90％（8／205）；CIN组治疗前单一高危HPV感染率最高，为52．55％（103／196），紧接着是混合高危感染23．97％（47／196）。③多重感染HPV的CIN患者治疗后3—6个月内HPV感染率未有明显的降低，而单一感染的患者治疗后3～6个月内HPV感染率显著下降。结论HPV多重感染不利于CIN治疗后患者的HPV的转阴。

Objective To explore the relationship between the patterns of HPV infection and turning-negative of HPV. Methods The subtypes and patterns of HPV infection after the treatment of CIN for 0,3,6 and 12 month in 196 CIN pa- tients and the 205 normal people were detected by PCR. Results ①Infection rates of CIN I , II ,III were 65.71% , 88.23% and 91.37%, each of which was higher than that in normal people. The Infection rates went higher with the CIN level increased. ②In the control group, single infection of high risk HPV contained the largest percentage-6.83% , fol- lowed by the multiple in infection of high risk HPV which was 3.90%. Similar condition was found in the CIN group,the rate of single and multiple infection of high risk HPV was 52.55% ,23.97%. ③The infection rate of multiple HPV infec- tion in patients after treatment for 3 - 6 months did not reduce, but the infection rate of single HPV infection decreased ob- viously. Conclusion Multiple HPV infection prevented HPV infection changing into negative.