反相高效液相色谱法测定麻仁丸中苦杏仁苷和芍药苷的含量

Determination on the Content of Amygdalin and Peoniflorin in MaRen Pills by RP-HPLC

目的：采用反相高效液相色谱法（reversedphasehigh-performanceliquidchromatography，RP_HPLC）测定麻仁丸中苦杏仁苷和芍药苷的含量。方法：色谱柱为AgilentTC—C18（4．6mm×250ram，5mm），流动相为乙腈-0．1％甲酸（11：89），检测波长为221nm，流速为1．0ml／min，柱温为30℃。结果：苦杏仁苷在0．2～2．0pg范围内呈良好线性关系，回归方程Y=0．1955X＋6．7365，r=0．9996，平均加样回收率为99．36％，RSD=1．3％（n=6）。芍药苷在0．2～2．0μg范围内呈良好线性关系，回归方程Y=0．1256X＋7．5868，r=0．9993，平均加样回收率为99．16％，RSD=2．00A（n=6）。结论：采用RP—HPLC法测定麻仁丸中苦杏仁苷和芍药苷，方法简便、准确、重现性好，为麻仁丸的质量控制和评价提供了一定的科学依据。

Objective:To apply the method of reversed phase high-performance liquid chromatography(RP-HPLC)for the content determination of Amygdalin and Peoniflorin in MaRen pills.Methods:The chromatographic column was Aglient TC-C18(4.6mm×250mm,5μm),the acetonitrile being 0.1%formic acid(11∶89),the flow rate 1.0ml/min,the detected wavelength221nm,and the column temperature was maintained at 30℃.Results:For Amygdalin the calibration curve was linear in the range of 0.2~2.0μg,r=0.9996.The linear equation was Y =0.1955 X+6.7365.The average recovery was 99.36%,RSD=1.3%(n=6).For Peoniflorin the calibration curve was linear in the range of 0.2~2.0μg,r=0.9993.The linear equation was Y =0.1256 X +7.5868.The average recovery was 99.16%,RSD=2.0%(n=6).Conclusion:The method is simple,accurate and reproducible and provides a scientific foundation for MaRen Pills quality control and evaluation.