奥拉西坦对大鼠脑缺血再灌注损伤Bcl-2及Bax表达的影响

Effect of Oxiracetam on Expression of Bcl-2 and Bax in Rats with Cerebral Ischemia-reperfusion Injury

目的探讨奥拉西坦对大鼠脑缺血再灌注损伤后Bcl-2及Bax蛋白表达的影响。方法利用大脑中动脉线栓法建立大鼠缺血再灌注模型,随机分为假手术组、生理盐水组、奥拉西坦小剂量组、奥拉西坦大剂量组。应用免疫组织化学法检测再灌注24 h后大鼠脑组织Bcl-2和Bax蛋白的表达。结果与假手术组比较,生理盐水组大鼠的Bcl-2和Bax蛋白表达明显增加(P<0.01),与生理盐水组比较,药物组Bcl-2表达显著增多(P<0.01),Bax表达显著减少(P<0.01),奥拉西坦大剂量组较奥拉西坦小剂量组的Bcl-2表达增多(P<0.05)、Bax表达减少(P<0.05)。结论局灶性脑缺血再灌注后Bcl-2、Bax表达增强,奥拉西坦能通过上调Bcl-2蛋白和下调Bax蛋白起到脑保护的作用。

Objective To investigate the effect of Oxiracetam on protein expression of Bcl-2 andBax in rats with cerebral ische-mia-reperfusion injury.Methods Rat ischemia-reperfusion model was established by using the middle cerebral artery suture method, rats were randomly divided into the shamoperative group,the saline group, the high-dose Oxiracetam group and the low-dose Oxirace-tamgroup .The Bcl-2 and Baxprotein expression were detected by using immunohistochemistry assay after 24hof reperfusion in rat brain tissue. Results Compared with thesham operative group, the rats＆#39;Bcl-2 and Bax protein expression was significantly in-creased in the saline group （P＆lt;0.01）, compared with the saline group, the rats＆#39;Bcl-2 expressionwas significantlyhigher in the drug group （P＆lt;0.01 ）, and the Bax expression was significantly reduced （P＆lt;0.01）, compared with low-dose Oxiracetam group, the rats’ Bcl-2 expression increased （P＆lt;0.05）, and the Bax expression decreased （P＆lt;0.05） in high-doseOxiracetam group.Conc lu-sion The expression of Bcl-2 and Bax were enhanced after focal cerebral ischemia and reperfusion.Oxiracetam can play a protective role inthe brain by up-regulating the Bcl-2 protein and down-regulating the Bax protein.