系统性红斑狼疮患者血清基质金属蛋白酶水平变化

Serum Matrix Metalloproteinases in Levels Patients with Systemic Lupus Erythematosus

目的探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者血清基质金属蛋白酶(matrix metalloproteinase,MMP)-3及MMP-9水平变化与疾病的关系和意义。方法测定SLE患者及健康对照者血清MMP-3,MMP-9水平,将SLE患者分为SLE活动期组与SLE非活动期组,且根据有无累及神经系统、肾脏及关节分为不同病损组,将同时无神经系统、肾脏及关节炎者定义为无器官损害组。结果共纳入SLE组患者85例,将其中临床资料相对充分的59例患者分为SLE活动期组22例,SLE非活动期组37例。SLE患者血清MMP-3水平明显高于对照组(P<0.01),SLE活动期和非活动期患者之间无明显差异(P>0.05);SLE活动期患者血清MMP-9水平低于SLE非活动期患者和对照组,差异有统计学意义(P<0.05,P<0.01)。神经系统损害组、肾损害组及关节炎组患者血清MMP-9水平明显低于无器官损害组(P<0.05)。结论血清MMP-3可能参与了SLE的发病,其水平下降可能与狼疮病情改善有关,可作为治疗后病情好转的信号。血清MMP-9水平和疾病活动或进行性脏器损害有关,有望作为评判SLE疾病活动和预后的指标。

To explore the serum matrix metalloproteinases levels of the patients with systemic lupus erythematosus in different disease courses. Methods We tested the serum matrix metalloproteinase-3 （ MMP-3 ） and matrix metalloproteinase-9 （MMP-9） of 85 patients and 30 healthy controls by ABC-ELISA （double antibody sandwich）. Results We found that the serum MMP-9 decreased significantly in patients with active disease while the those in patients with stable disease and healthy controls were not changed （P〈0.05 and P 〈0. 01, respectly）. The serum MMP-3 changed in the opposite way, the MMP-3 level was higher in patients with SLE than healthy controls （ P 〈 0.01 ）. The serum levels of MMP-9 were significantly decreased in patients without renal involvement, nervous system involvement or arthritis when compared to patients with all these organs involvement （P 〈 0. 05）. Conclusions The matrix metalloproteinases may have different effects on different disease phases of SLE and are related to active diseases. The change of the matrix metalloproteinases may be contribute to the pathogenesis of SLE.