55株小单孢菌对西罗莫司的微生物转化

Microbial conversion of sirolimus by 55 strains of Micromonospora sp.

从55株小单孢菌转化西罗莫司的研究中获得了15株能够转化西罗莫司的小单孢菌。其中,13株小单孢菌转化西罗莫司产生7个产物,它们在HPLC图谱中有相同的相对保留时间。在另2株菌中,我们选出小单孢菌FIM02-848进行深入研究,经分类学研究鉴定为棘孢小单孢菌FIM02-848,该菌株转化西罗莫司产生7个转化产物,我们从中分离获得了两个转化产物,一个分子量为916的转化产物进行结构鉴定表明,它与14-羟基雷帕霉素同质;另一个分子量为900的转化产物进行HPLC、UV和MS分析,均显示它与14-去氧雷帕霉素具有相同的特性,推测它可能是14-去氧雷帕霉素。

In the research of microbial conversion of rapamycin by 55 strains of Micromonospora sp., among them, 15 strains were found to be capable of transforming rapamycin into various products. By HPLC analyse, it showed of 13 strains could transform rapamycin into 7 products with the same relative retention time. Micromonospora sp. FIM02-848 was selected for further study and classified as Micromomospora echinospora FIM02-848, the strain could transform rapamycin into 7 products. One converted product（RRT 0.92） with molecular weight of 916 was identified with 14-hydrorapamycin. The other converted product（RRT 1.78） with molecular weight of 900 showed it was the same as 14-deoxorapamycin in HPLC, UV and MS. Thus, it was inferred to be 14-deoxorapamycin.