盐酸川芎嗪脂质体的制备及其理化性质的表征

Preparation and characterization of ligustrazine hydrochloride liposomes

目的制备盐酸川芎嗪脂质体,并考察其包封率、载药量、粒径、电位、体外释放性等理化性质。方法用主动载药法制备盐酸川芎嗪脂质体,用透析法测定包封率和载药量,用激光粒度及Zeta电位分析仪测粒径和电位,用透析释放试验考察脂质体的体外释放性。结果盐酸川芎嗪脂质体的平均包封率最高可达81.2%,平均载药量为4.05%,平均粒径为173.4 nm,Zeta电位为11.5 mV。结论制备的盐酸川芎嗪脂质体的包封率高且粒径均一,电位稳定,体外释放完全。

OBJECTIVE To prepare and characterize ligustrazine hydrochloride liposomes. METHODS The ligustrazine hydro- chloride liposomes were prepared using ammonium sulphate gradients method. The ligustrazine hydrochloride liposomes were character- ized with particle size, Zeta potential, entrapment efficiency, drug loading and the stability. RESULTS The encapsulation rate of ligus- trazine hydrochloride liposomes reach up to 81.2% , drug loading was 4.05% , average size was about 173.4 nm, and the Zeta - potential was about 11.5 mV. CONCLUSION The liposomes show high encapsulation efficiency with a small and uniform particle size, and stable Zeta - potential.