HPLC单波长同时测定六味甘草丸中的甘草苷和甘草酸铵

Simultaneous determination of liquiritin and glycyrrhizic acid ammonium in six kinds of licorice pill by single wavelength HPLC method

目的采用HPLC法单波长同时测定六味甘草丸中甘草苷和甘草酸铵的含量。方法采用EClipseXDB-C18色谱柱（250mm×4．6mm，5μm），柱温30℃，流动相A为乙腈、流动相B为0．05％磷酸溶液，梯度洗脱，检测波长为237nm；理论板数按甘草苷峰计算应≥5×10^3。结果甘草苷和甘草酸铵的线性范围分别为0．025～0．250μg（r=0．9999）、0．208～2．080μg（r=0．9998），平均样回收率分别为99．78％、99．33％，RSD分别为1．19％、1．41％（n=6）。结论所用方法准确、简便、灵敏、可靠，可用于该制剂的质量控制。

OBJECTIVE To establish the determination of liquiritin and glycyrrhizic acid ammonium in six kinds of licorice pill by single wavelength HPLC method. METHODS The chromatographic column was EClipse XDB - C18 column （ 250 mm × 4.6 mm, 5 μm） ,with the temperature of 30 ℃ ,and acetonitrile as mobile phase A,0.05% phosphoric acid solution as mobile phase B. The detec- tion wavelength was 237 nm. The number of theoretical plates should not be less than 5 × 10^3 according to the calculation of liquiritin peak. RESULTS The linear range of liquiritin linear range was 0.025 - 0. 250 p.g （ r = 0. 9999 ）, the average recovery was 99.78% with RSD of 1.19 % （ n = 6 ） ; The linear range of ammonium glycyrrhizinate was 0. 208 - 2. 080 μg （ r = 0. 9998 ） , the average recovery was 99.33% ,RSD = 1.41% （n = 6）. CONCLUSION The method is accurate, simple, sensitive, reliable, and can be used for the quality control of the preparation.