四川白魔芋球茎中甘露聚糖酶部分酶学性质

Some properties of β-mananase from the tubers of Αmorphophallus albus

从萌发的四川白魔芋球茎中分离纯化β -甘露聚糖酶 ,该酶的最适 p H为 5.1,最适温度为 50℃ ,有较好的热稳定性和较宽的 p H适宜范围 ( 5.0～ 9.0 ) ,其米氏常数 Km =5.4 9× 10 - 2 %。该酶可将球茎中所含的甘露聚糖降解成二糖、三糖、四糖和五糖等水溶性的甘露低聚糖。

mananase hydrolyzing konjac mannan into glucomannan oligosaccharides was extracted and purified from germinating Amorphophallus albus tubers. The pH value and temperature for optimum catalysis of the enzyme were 5.1 and 50 ℃, respectively. The K m  constant of the enzyme for Amorphophallus albus mannan was 5.49×10 -2 %.