华茶藨组织培养及试管苗繁殖的研究

Study on Tissue Culture and Tube Seedlings' Propagation of Ribes fasciculatum

[目的]对华茶藨组织进行培养,并研究试管苗的繁殖。[方法]以华茶藨为材料,采用嫩茎组织培养方法,进行愈伤组织诱导、增殖、分化培养,以及试管苗生根、移栽、定植的研究。[结果]MS+35 g/L蔗糖+0.4 mg/L NAA+0.4 mg/L ZT+1.8 mg/L 2,4-D为嫩茎愈伤组织诱导培养的适宜培养基;MS+1.6 mg/L 2,4-D+0.6 mg/L 6-BA+0.3 mg/L NAA的固态培养基是愈伤组织增殖培养的适宜培养基;MS+0.5 mg/L ZT+0.1 mg/L NAA为愈伤组织分化培养和不定芽繁殖培养的适宜培养基。1/2MS+0.6 mg/L IAA+0.1 mg/L NAA+16 g/L蔗糖为试管苗培养和试管苗繁殖培养的适宜培养基。试管苗移栽成活率为96.7%;试管苗定植成活率为98.6%。[结论]定植苗次年可5月开花,10月结果,其他植物学性状均与野生华茶藨一致。

[ Objective] To conduct tissue culture of Ribesfasciculatum and Study propagation of tube seedlings. [ Method l With the method of tissue culture, the tender stems of Ribes fasciculatum were used as materials to do the research on callus inducement, propagation and differen- tiation; cultivation, transplantation of tube seedlings and stable planting. [ Result] The results demonstrated that MS ＋ sucrose 35 g,/L ＋ 0.4 mg/L NAA ＋ 0.4 mg/L ZT ＋ 1.8 mg/L 2,4-D was the ideal medium for callus inducement ; MS ＋ 1.6 mg/L 2,4-D ＋ 0.6 mg/L 6-BA ＋ 0.3 mg/L NAA was the ideal medium for callus multiplication; MS ＋ 0.5 mg/L ZT ＋ 0.1 mg/L NAA was the optimum medium for callus differen- tiation cultivation and adventitious buds cultivation ; The ideal medium for cultivation and propagation of tube seedlings was 1/2MS ＋ 0.6 mg/L IAA ＋0.1 mg/L NAA ＋ 16 g/L sucrose. The transplanting survival rate of tube seedlings was 96.7% and stable planting survival rate was 98.6%. [ Conclusion] The flowering of the tube seedlings stable planted the second year can be normal and it maintained all botany character- istics of the wild Ribes fasciculatum.