化妆品中甲醛毒性的发光细菌测定法研究

Determination of toxicity of formaldehyde in cosmetics by the method using luminescent bacteria Virbrio qinghaiensis sp.-Q67

以发光细菌(青海弧菌Q67)作为检测活体检测化妆品中甲醛的毒性。通过检测发现随着甲醛质量浓度的升高和作用时间的延长,青海弧菌Q67的相对发光强度逐渐降低;作用时间在15 min时,甲醛对青海弧菌Q67的效应拟合曲线线性较好,甲醛对青海弧菌Q67的半效应浓度为0.033 g·L-1;甲醛对青海弧菌Q67的最低检测限为0.01 g·L-1。

Luminescent bacteria Virbrio qinghaiensis sp. -Q67 was used as detection living body to assess the toxicity of formaldehyde in cosmetics. Via the determination test, it was found that following the increase of mass concentration of formaldehyde as well as the extension of action time, the relative luminous intensity of Virbrio qinghaiensis sp. - Q67 reduces gradually. Results showed that when the action time is 15 min, the fitting curve for effect of formaldehyde on Virbrio qinghaiensis sp. - Q67 displays better linearity. The half - efficient concentration value is 0. 033 g ·L^-1, The low detection limit value is 0. 01 g ·L^-1.