五味子乙素对UVA损伤组织工程皮肤保护作用的研究

Research on effects of Sanhedrin B protective effect on the long wave ultraviolet damage tissue engineering skin

目的:探讨五味子乙素(Sch B)对长波紫外线(UVA)接种人永生化角质形成细胞(Ha Cat细胞)和成纤维细胞(FB细胞)的组织工程皮肤模型损伤后的保护作用及其可能的作用机制。方法:用5J/cm2的UVA照射组织工程皮肤模型,给予不同浓度的Sch B(0.01、0.1、1、10、100μmol/L)处理UVA损伤后的组织工程皮肤模型,检测细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)的活性,乳酸脱氢酶(LDH)及一氧化氮(NO)含量。结果:UVA照射组织工程皮肤模型后,SOD、GSH-PX活性降低,LDH及NO含量升高,给予不同浓度的Sch B对SOD、GSH-PX活性、LDH及NO含量,有不同的影响,实验结果表明以0.01、0.1μmol/L Sch B的保护做用最强。结论:0.01、0.1μmol/L Sch B减轻UVA对组织工程皮肤模型损伤的效果最佳。

Objective The aim to explore the effect of Sanhedrin B （Sch B） on tissue engineering skin injury caused by the long wave ultraviolet （UVA） and its possible Mechanism.Methods Tissue engineering skin was irradiated with a dose of 5 J/cm2 UVA,treated with different concentrations of Sch B （0.01,0.1,1,10,100μmol/L） treatment after UVA damage of tissue engineering skin,the detection cell superoxide diastase （SOD） and glutathione peroxidase （GSH-PX） activity,lactate dehydrogenase （LDH） and nitric oxide （NO） content.Results After tissue engineering skin was irradiated by UVA,the result of which reduced the SOD,GSH-PX activity,increased LDH and NO content,treated with different concentrations of Sch B,which could affect the SOD,GSH,LDH,NO activity,to improve the survival rate tissue engineering skin,especially with the protection of the 0.01,0.1μmol/L Sch B do with strongest.Conclusion 0.01,0.1μmol/L Sch B can be a certain extent and reduce the UVA damage of tissue engineering skin.