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五味子乙素对UVA损伤组织工程皮肤保护作用的研究 被引量:7

Research on effects of Sanhedrin B protective effect on the long wave ultraviolet damage tissue engineering skin
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摘要 目的:探讨五味子乙素(Sch B)对长波紫外线(UVA)接种人永生化角质形成细胞(Ha Cat细胞)和成纤维细胞(FB细胞)的组织工程皮肤模型损伤后的保护作用及其可能的作用机制。方法:用5J/cm2的UVA照射组织工程皮肤模型,给予不同浓度的Sch B(0.01、0.1、1、10、100μmol/L)处理UVA损伤后的组织工程皮肤模型,检测细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)的活性,乳酸脱氢酶(LDH)及一氧化氮(NO)含量。结果:UVA照射组织工程皮肤模型后,SOD、GSH-PX活性降低,LDH及NO含量升高,给予不同浓度的Sch B对SOD、GSH-PX活性、LDH及NO含量,有不同的影响,实验结果表明以0.01、0.1μmol/L Sch B的保护做用最强。结论:0.01、0.1μmol/L Sch B减轻UVA对组织工程皮肤模型损伤的效果最佳。 Objective The aim to explore the effect of Sanhedrin B (Sch B) on tissue engineering skin injury caused by the long wave ultraviolet (UVA) and its possible Mechanism.Methods Tissue engineering skin was irradiated with a dose of 5 J/cm2 UVA,treated with different concentrations of Sch B (0.01,0.1,1,10,100μmol/L) treatment after UVA damage of tissue engineering skin,the detection cell superoxide diastase (SOD) and glutathione peroxidase (GSH-PX) activity,lactate dehydrogenase (LDH) and nitric oxide (NO) content.Results After tissue engineering skin was irradiated by UVA,the result of which reduced the SOD,GSH-PX activity,increased LDH and NO content,treated with different concentrations of Sch B,which could affect the SOD,GSH,LDH,NO activity,to improve the survival rate tissue engineering skin,especially with the protection of the 0.01,0.1μmol/L Sch B do with strongest.Conclusion 0.01,0.1μmol/L Sch B can be a certain extent and reduce the UVA damage of tissue engineering skin.
出处 《中国美容医学》 CAS 2014年第6期457-461,共5页 Chinese Journal of Aesthetic Medicine
基金 国家自然科学基金资助项目(项目编号:81072964)
关键词 五味子乙素 紫外线 组织工程皮肤 Sanhedrin B ultraviolet rays tissue engineering skin
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参考文献14

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