禿杉组织培养研究

STUDIES ON THE TISSUE CULTURE OF TAIWANIA FLOUSIANA

本文详细报道了从秃杉(Taiwania flousiana Gaussen)离体胚诱导不定芽、不定根及从无菌苗茎端培养再生植株的过程。诱导不定芽要求较低的蔗糖浓度(以3％最好);同时BA是必须的,在附加0.1—3 mg/1 BA的White培养基上,从离体胚的子叶或胚轴上诱导了不定芽的发生(以1 mg/1最好);NAA与BA结合使用,对不定芽诱导无促进作用;适当提高光照有利于不定芽的诱导。在诱导不定芽的同时,在子叶表面还观察到有许多无结构的“不定突起”。不定芽起源于子叶表皮下1—2层细胞。IBA对诱导离体胚上产生不定根效果较好。在有或无生长素的培养基上,从生长1月龄的无菌苗茎端培养获得了不定根的产生,在加有细胞分裂索的培养基上,从无菌苗上产生了腋芽。

The present paper reports the processes that adventitious buds and roots are induced from excised embryos and plantlets are regenerated from bacteria-free shoots of one-month-old seedling of Taiwania ftousiana Gaussen. For inducing of adventitious buds, BA is necessary and sucrose of lower concentration is required (the best concentration of BA is 1 mg/l, sucrose's is 3%), and increasing light intensity properly is adventage. Adventitious protuberance is seen when adventitious bud is induced. The best auxin to induce adventitious roots from the excised embryos is IBA among IBA, IAA, NAA, and 2, 4-D. Bacteria-free shoots of one-month-old seedlings are induced to form adventitious roots when cultured on media containing auxin or not and to develop axillary buds when cultured on media containing cytokinin. Cytohistological examination showed that the adventitious buds originated from cells of 1st to 2nd layer under epidermis of the cotyledon.