芦荟内生菌内生哈茨木霉LH-7对植物病原菌的抗性

Inhibition effects and mechanisms of the entophytic fungus Trichoderma harzianum LH-7 from Aloe barbadensis

通过体外培养方法，研究了药用植物库拉索芦荟内生真菌内生哈茨木霉LH-7对9种常见植物病原菌的抑菌谱及其拮抗机理．结果表明：内生哈茨木霉LH-7对供试植物病原真菌具有显著的抑制作用（抑制率为62．4％～88．4％），且营养竞争和重寄生是其主要拮抗机制．该菌株的代谢产物中具有能够抑制病原菌生长及孢子萌发的活性物质，其作用可导致病原菌菌丝体生长的畸形、细胞壁破裂、产孢结构发育不健全、孢子形成及萌发率降低，从而有效抑制病原菌的生长和繁殖．

Inhibition spectrum and antagonistic mechanism of an endophytic fungus Trichoderma harzianum LH-7, isolated from wild medicinal plant Aloe barbadensis, were investigated by in vitro culture methods against 9 kinds of plant pathogens. The results showed that nutrient competition and hyper-parasitism were the two primarily antagonist approaches that strain LH-7 adopted to inhibit the tested plant pathogens with a significant inhibition rate of 62.4% -88.4%. Moreover, the active compound from metabolites of LH-7 could cause pathogen mycelial deformities, cell wall rupture and conidial malformation, leading to the effective inhibition on pathogens growth and reproduction.