一种离体皮肤经皮电阻测量装置的研制

Development of a novel device for detection of transcutaneous electrical resistance in vitro

目的 研制一种能固定离体皮肤和稳定测量经皮电阻值的离体皮肤经皮电阻测量装置.方法 离体皮肤经皮电阻测量装置由离体皮肤固定装置和经皮电阻测量仪组成,皮肤固定装置固定离体皮肤,固定装置装有电解质溶液,皮肤与内外之间的电解质溶液形成电导池,电阻测量仪通过皮肤固定装置中的内外电极,测量离体皮肤的经皮电阻值.取同一只Wistar大鼠背侧部的8块正常离体皮肤固定于离体皮肤固定装置中,测量并记录18个时间点的经皮电阻值,以上实验重复3次.结果 检测结果在正常值范围内,3次重复实验的经皮电阻值变化曲线基本一致,说明该装置能维持离体皮肤在24 h内屏障功能的稳定.结论 离体皮肤经皮电阻测量装置能满足皮肤刺激性和（或）腐蚀性试验离体皮肤替代方法的实验要求,还能用于其他有关药物的释放和透皮吸收试验的体外方法,以及测量经皮电阻值的相关方法.

Objective To develop a novel device for in vitro fixation of skin and measurement of transcutaneous electrical resistance.Methods This device was consisted of in vitro skin fixation apparatus and an instrument for measuring electrical resistance.The fixation apparatus was filled with electrolyte,thus allowing for the formation of a conductivity cell between the inner and outer surface of skin,which measured the transcutaneous electrical resistance via the inner and outer electrodes.Following fixation to the apparatus,eight normal skin tissues derived from a Wistar rat received,the measurement of transcutaneous resistance at 18 time points,which was repeated for three times.Results The measured data were within the normal range and three repeated experiments showed consistency in the skin resistance curves suggesting that this device was capable of maintaining the in vitro stability of skin barrier function within 24 hours.Conclusion The newly developed device satisfies the requirements of alternative methods for in vitro skin irritation/corrosion tests and can be applied for in vitro drug release,transcutaneous absorption tests and the measurement of transcutaneous electrical resistance.