结直肠癌中Jab1的表达及其预后意义

Expression and significance of c-Jun activation domain binding protein 1 in human colorectal carcinoma

目的 探讨c-Jun激活区结合蛋白1（Jab1）在结直肠癌发生发展中的作用及其与结直肠癌临床病理特征的关系.方法 回顾性收集嘉兴市第一医院2007年1月至2008年12月行手术切除的80例结直肠癌组织标本,采用免疫组织化学EnVision二步法检测结直肠癌及其相应癌旁正常组织中Jab1的表达情况并分析其与临床病理特征的关系;提取6例新鲜结直肠癌手术标本的总蛋白并进行定量,通过蛋白质印迹检测结直肠癌组织与癌旁正常组织中Jab1的表达.结果 免疫组织化学及蛋白质印迹结果均显示,Jab1在结直肠癌组织中的表达显著高于在癌旁正常组织中的表达,结直肠癌组织中Jab1阳性表达率为96.3％ （77/80）,高表达者66例（82.5％）,低表达者14例（17.5％）;而癌旁组织中Jab1阳性表达率为8.8％ （7/80）,与结直肠癌组织之间差异有统计学意义（P ＜0.05）;Jab1阳性表达与肿瘤细胞分化程度、浸润深度、TNM分期和淋巴结转移情况有关（均P＜0.05）;Jab1阳性表达与增殖指标Ki-67表达呈显著正相关（r=0.548,P＜0.01）,与p27kip1表达呈显著负相关（r=-0.461,P＜0.01）.结论 Jab1异常表达可能参与了结直肠癌的发生发展并与p27kip1负相关.Jab1有望成为评价结直肠癌恶性程度和判断预后的重要参考指标.

Objective To explore the association of Jab1 （c-Jun activation domain binding protein 1） expression during carcinogenesis and clinicopathological characteristics of colorectal carcinoma （CRC）.Methods Tissue specimens were obtained from 80 cases of CRC from January 2007 to December 2008.And the expression of Jab1 protein for each specimen was detected by immunohistochemistry （EnVision）.Six representative paired samples of cancerous and paired adjacent normal tissues were collected for Western blot.The relationships between the expression level of Jab1 protein and the clinicopathological characteristics of primary CRC were retrospectively analyzed.Results A high-level expression of Jabl was present in cancerous tissues but not in paired adjacent normal tissues.The positive expression rate of Jab1 protein was as high as 96.3% （77/80）.And its high expression rate was 82.5% （66/80）,low expression rate 17.5% （14/80）and 8.8% （7/80）in cancerous and paired adjacent normal tissues respectively （P 〈 0.05）.Its expression was correlated with differentiation,invasion depth,TNM stage and lymph node metastasis （all P 〈 0.05）.Jab1 was significantly correlated with Ki-67 （r =0.548,P 〈 0.01） and inversely with p27kipl （r =-0.461,P 〈 0.01）.Conclusions An over-expression of Jabl protein might play an important role in the pathogenesis of CRC.Thus it may become a novel diagnostic marker and a therapeutic target in patients with CRC.