通关藤化学成分HPLC指纹图谱研究

HPLC Fingerprint of Chemical Constituents of Marsdenia tenacissima

目的：研究通关藤化学成分的指纹图谱.方法：采用HPLC以TC-C18色谱桂（4.6 mm× 150 mm,5 μm）,乙腈-水为流动相进行梯度洗脱,检测波长210 nm,流速1.0 mL· min-,柱温25℃.结果：以ZP-7通关藤苷元B（[11 α-O-（2-甲基丁酰基）-12β-O-乙酰基通关藤苷元B]）为参照物,建立了通关藤的指纹图谱,指认了17个共有峰,精密度和重复性中共有峰相对保留时间RSD＜ 3％,每个产地色谱图中的共有峰的峰面积之和＞90％,10批药材的相似度在0.75 ～0.98,存在一定差异.结论：该方法准确、重复性好,为通关藤质量评价和质量控制提供依据,也为通关藤进一步开发奠定基础.

Objective：Fingerprint analysis of chemical constituents of Marsdenia Tenacissima.Method：With the use of HPLC,TC-C18 column （4.6 mm × 150 mm,5 μm）,acetonitrile-water was used as the mobile phase with gradient elution,detection wavelength at 210 nm,flow rate of 1.0 mL ·min-1,temperature of column at 25 ℃.Result：The fingerprint of M.tenacissima was established using ZP-7tenacigenin B [11α-O-（2-methylbutyryl）-12β-O-acetyltenacigenin B] as reference substance.17 common peaks were identified.The precision and repeatability of the relative retention time RSD were less than 3％,each producing area in the chromatogram of common peak area was more than 90％.10 batches of similarity were between 0.75-0.98,there were some differences.Conclusion：The method is accurate and reproducible,which can provide the basis for quality evaluation and quality control of M.tenacissima,also lay the foundation for further development of M.tenacissima.