HPLC测定哈蟆油中4-胆甾烯-3-酮及胆固醇含量

Determination of 4-Cholesten-3-one and Cholesterol in Oviductus Ranae by HPLC

目的：采用高效液相法同时测定哈蟆油中4-胆甾烯-3-酮和胆固醇.方法：使用Agilent TC-C18柱（4.6 mm×250 mm,5μm）,流动相甲醇-永（91.5∶8.5）,柱温35℃,体积流速1.0 mL·min-1,检测波长215 nm,进样量10 μL.结果：哈蟆油中4-胆甾烯-3-酮进样量在0.011 65～0.116 5μg呈良好的线性关系,平均回收率为100.0％,RSD 1.83％ （n =6）,且稳定性、精密度及重复性均良好;胆固醇进样量在2.282～22.82 μg成良好的线性关系,平均回收率为99.3％,RSD 1.19％（n=6）,且稳定性、精密度及重复性均良好.结论：该方法能够同时、准确、稳定、快速的测定哈蟆油中4-胆甾烯-3-酮和胆固醇的含量.

Objective：To determine the content of 4-cholesten-3-one and cholesterol in Oviductus Ranae by HPLC at the same time.Method：Agilent TC-C18 column （4.6 mm ×250 mm,5 μm） was used.The mobile phase consisted of methanol-water （91.5∶ 8.5）.The column temperature was maintained at 35 ℃.The volumetric flow rate was set at 1.0 mL ·min-1.The UV detector was set at 215 nm.The sample size was 10 μL.Result：4-cholesten-3-one was linear in the content range of 0.011 65-0.116 5 μg; the average recovery was 100.0％,RSD 1.83％.Cholesterol was linear in the content range of 2.282-22.82 μg; the average recovery was 99.3％,RSD 1.19％.Conclusion：The method,which is fast,accurate and stable,can simultaneously determine the content of 4-cholesten-3-one and cholesterol in Ranae Oviductus.