摘要
目的:观察参芪益心方对慢性心力衰竭(CHF)大鼠心肌形态、心功能、血浆中血管紧张素Ⅱ(AngⅡ)、肿瘤坏死因子-α(TNF-α)的影响。方法:将90只Wistar雄性大鼠随机平均分为6组:正常对照组、模型组、依那普利对照组、芪苈强心对照组、参芪益心方低剂量(16.96 g·kg-1·d-1)组、高剂量(33.92 g·kg-1·d-1)组。利用阿霉素第l,3,5周,3 mg·kg-1;第2,4,6周,2 mg·kg-1的剂量,每日1次,灌胃4周,观察大鼠的心肌组织形态学及超微结构变化,运用超声心动图检测大鼠左室收缩末期内径(LVSD)、左室舒张末期内径(LVDD)、左室射血分数(EF)、左室短轴缩短率(FS),放免法测定大鼠血浆AngⅡ,TNF-α。结果:经EF,FS提示造模成功,各治疗组LVSD,LVDD,EF,FS均有改善,参芪益心方高剂量组、中药对照组、西药对照组与模型组比较有统计学意义(P<0.05)。各治疗组AngⅡ,TNF-α均有不同程度的降低,与模型组比较有统计学意义(P<0.05)。结论:参芪益心方可以改善CHF大鼠心肌组织形态,抑制心室重构,改善CHF大鼠心功能。
Objective:To observe the effect of the Shenqi Yixin Fang on cardiac function,plasma angiotensin Ⅱ (Ang Ⅱ) and tumor necrosis factor-α (TNF-α) of chronic heart failure (CHF) rats.Method:Ninty male Wistar rats were randomly divided into six groups:normal control group,model group,enalapril control group,the Qili Qiangxin control group,the low dose of Shenqi Yixin Fang group (16.96 g ·kg-1·d-1),the high dose of Shenqi Yixin Fang group (33.92 g ·kg-1·d-1).Doxorubicin was used to induce CHF models,and the modelling rats were given drug intervention for 4 weeks.The echocardiography was used to measure cardiac function,plasma Ang Ⅱ and TNF-α were measured by radioimmunoassay.Result:In model group the left ventricular end-systolic diameter (LVSD),left ventricular end-diastolic diameter (LVDD),left ventricular ejection fraction (EF),left ventricular fractional were shorten compared with normal control group (P < 0.05).In treatment group,these indicators were improved compared with modek group (P < 0.05).In treatment groups the plasma Ang Ⅱ/TNF-α levels were reduced compared with the model group (P < 0.05).Conclusion:The Shenqi Yixin Fang can improve myocardial tissue morphology,inhibit ventricular remodeling and improve cardiac function in CHF rats.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期128-132,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
黑龙江省自然科学基金(QC2010091/C190402)
黑龙江省教育厅科学技术研究项目(12531615)
黑龙江中医药大学新药基金(201102)