EAE小鼠中枢神经系统中CD11b mRNA的变化及益肾达络饮对其的影响

Expression of CD11b mRNA in Central Nervous System and Effect of Yishen Daluo Decoction in Experimental Autoimmune Encephalomyelitis

目的:观察实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)小鼠不同发病时期脑和脊髓组织中CD11b mRNA的表达变化,明确益肾达络饮的干预机制。方法:采用MOG35-55抗原免疫C57BL/6小鼠诱导EAE模型,随机选取分为正常组,佐剂组、模型组、醋酸泼尼松3.9 mg·kg-1组、益肾达络饮20 g·kg-1组,在造模后第7天开始灌胃给药,直到相应时间点进行取材。采用实时荧光定量PCR测定免疫后7 d(发病前期)、14 d(发病初期)、24 d(发病急性期)、40 d(慢性持续期)4个不同时间点EAE小鼠脑和脊髓组织中CD11b mRNA表达的变化。结果:与正常组比较,在免疫后14,24,40d,模型组EAE小鼠大脑中CD11b mRNA表达分别升高至2.39±1.09,3.20±1.14,1.58±0.36(P<0.05)。与模型组比较,在免疫后40 d激素组和益肾达络饮组EAE小鼠大脑中CD11b mRNA的表达水平明显降低(P<0.05);与正常组比较,激素组、益肾达络饮组在免疫后40 d CD11b mRNA在EAE小鼠大脑中表达水平无显著改变;在免疫后14 d激素组EAE小鼠脊髓中CD11b mRNA的表达水平明显降低。结论:单核细胞是实验性自身免疫性脑脊髓炎的潜在调节细胞;中药复方益肾达络饮干预EAE的作用机制与中枢神经系统单核细胞黏附分子CD11b的调节密切相关。

Objective：To elucidate the CD11b mRNA expression of brain and spinal cord tissue during the different phrase in experimental autoimmune encephalomyelitis （EAE） mice,and to explore the mechanism of Yishen Daluo decoction （YSDLD） on EAE.Method：C57BL/6 mice were immunized with the MOG35-55.Mice were divided randomly into normal-group,adjuvant group,EAE-group,prednisone-group,YSDLD-goup.RT-PCR was used to quantify the gene expression levels of immune/inflammatory cell （the monocyte marker CD11b）,accumulated in brain and spinal cord tissue of mice at different time points of EAE including pre-onset （on day 7th）,onset （on day 14th）,peak （on day 24th） and chronic phrase （on day 40th） after immunization.Result：Compared with model group,CD11b mRNA levels in the cerebrum were significantly decreased 40 days after immunization in hormone-treated group and YSDLD-treated group.CD11b mRNA levels in the spinal cord were significantly decreased 14 days after immunization in hormone-treated group.Conclusion：The monocyte is one of potential mediators of EAE.The therapeutic mechanism of YSDLD is to mediate the expression of CD11 b on the surface of monocyte.