新型去甲万古霉素-纤维蛋白胶磷酸钙缓释系统中抗菌素的体外释放规律

The releasing raw of the antibiotic in vitro in a new norvancomycin-fibrin glue calcium phosphate bone substitute

目的：通过对比两种磷酸钙人工骨中去甲万古霉素在体外的释放规律,为新型载药缓释系统的临床应用提供实验依据。方法：将适量去甲万古霉素粉末以生理盐水充分溶解,制成一定浓度抗菌素溶液,逐步稀释形成去甲万古霉素标准液的浓度梯度,在一定温度下,以紫外分光光度计在200-800 nm波段间进行连续扫描,取最大吸收峰值处波长为以后测量的参考波长,将各浓度下测得的光密度（optical density下称OD值）导入SPSS13.0软件包中,计算溶液标准回归方程并检验之。分别将一定量新制备的两组材料试件投于生理盐水中持续浸提,定期更换试管取浸提液,在上述参考波长下测其光密度,代入方程计算各期浸提液中去甲万古霉素的浓度及累计释放量。结果：去甲万古霉素溶液在25℃条件下于226 nm波长处出现最高峰,OD值随浓度x（mg·L-1）的标准回归方程为：OD=0.013x＋0.173（R2=0.971,调整R2=0.964,方程检验P=0.00）,实验组维持有效浓度可达6周以上,较对照组明显延长。结论：纤维蛋白胶可明显延长载药缓释体系中抗生素的释放时间,早期遵循Higuchi＇s规律。

Objective： By comparing of the release law of norvancomycin in two calcium phosphate bone substitute in vitro, we aim to provide experimental evidence for clinical application of the new-type drug delivery system. Methods： Norvancomycin powder was fully dissolved in saline to make a certain concentration, and gradually diluted to form a gradient of norvancomycin. UV spectrophotometer was applied to successively scan at 200 - 800 nm bands at a certain temperature, and the wavelength of maximum absorption peak was considered as the reference wavelength, every concentrations measured were absorhanced into SPSS13.0 package, calculated and tested the regression equation of the standard solution. Respectively, a certain amount of the two new-made material was put into saline and extracted continously, regular changed the tube to take extracts,measured its optical density（ OD value） at the reference wavelength absorhance above, and all OD values in each period were substituted into the equation and concentrations of norvancomycin and the total emissions were calculated. Results ： Under room condition of 25 ℃, standard NV solution had a maximum absorption peak at a wavelength of 226 nm. The relationship between OD value and concentration x （mg · L^-1） of the standard NV solution can be described as ： OD = 0. 013x ＋ 0. 173 （ R2 = 0. 971, adjusting R2 = 0. 964,P = 0.00）. In Experimental Group an effective concentration could last for 6 weeks, significantly longer than that in the Control Group. Conclusions： Fibrin glue can significantly prolong the drug release system for antibiotic release time, and follow the law of Higuchi＇s in early period.