RP-HPLC法测定五味子中三种成分的含量

Content determination of three compositions in fructus schisandrae by RP-HPLC

目的:建立反向高效液相色谱法测定五味子中五味子醇甲、五味子甲素、五味子乙素含量的方法。方法:采用SUNTEK Kromasil C18柱(4.6 mm×250 mm,5μm),Dikma Kromasil C18柱(4.6 mm×250 mm,5μm),以水-甲醇梯度洗脱为流动相,流速:1 mL/min,检测波长:254 nm。结果:五味子醇甲、五味子甲素、五味子乙素进样量均在0.02μg^1.0μg范围内与峰面积有良好线性关系,r2=0.999 9、1.0、0.999 8;平均回收率分别为98.5%、98.1%、98.6%;RSD分别为1.2%、1.4%、2.4%(n=9)。结论:该方法方便、准确、专属性好,适用于五味子的质量控制。

Objective：To establish a method for content determination of schizandrin,schizandrin A and schizandrin B in fructus schizandrae by RP-HPLC. Methods：HPLC was performed on SUNTEK Kromasil C18 column （4.6 mm×250 mm,5μm）and Dikma Kromasil C18 column （4.6 mm×250 mm,5μm）with water and MeOH （gradient elution） as mobile phase. Flow rate was 1.0 mL/min and detection wave length was 254 nm. Results：The linear relationship of schizandrin,schizandrin A and schizandrin B was good in the range of 0.02μg^1.0μg with correlation coefficients 0.999 9、1.0、0.999 8. The average recovery rate was 98.5%、98.1%、98.6%and RSD was 1.2%、1.4%、2.4%（n=9）. Conclusion：The method is convenient,ac-curate,specific and suitable for the quality control of fructus schizandrae.