25例肾病综合征患儿调节性T细胞水平变化及意义

The changes and clinical significance of regulatory T cells in 25 children with nephrotic syndrome

目的观察肾病综合征(NS)患儿外周血中调节性T细胞(Treg)、淋巴细胞各亚群的变化,探讨其在疾病发生和发展中的作用。方法选取20例健康儿童作为对照组,25例患儿作为肾病综合征组。采用流式细胞术对两组儿童进行外周血Treg、淋巴细胞各亚群检测。结果与正常对照组比较,NS组的外周血CD4+CD25+CD127-/low Treg细胞、CD3+CD4+辅助性T细胞、CD19+B细胞表达均明显降低(P<0.05);而CD3+CD8+抑制性T细胞表达明显升高(P<0.05);肾病综合征组患儿的CD16+CD56+NK细胞表达降低,但与对照组相比无统计学差异(P>0.05)。结论淋巴细胞免疫功能的紊乱,特别是Treg的表达及其功能的改变,参与了肾病综合征的发病,其检测可有助于肾病综合征的诊治。

Objective To observe the changes of regulatory T cells （Treg） and lymphocyte subsets in peripheral blood in chil- dren with nephrotic syndrome （NS）, and to discuss its role in the occurrence and development of the disease. Methods Twenty healthy children were set as control group. Twenty-five children with nephrotic syndrome were set as NS group. The expressions of Treg and lymphocyte subsets in peripheral blood were analyzed by flow cytometry. Results Compared with control group, the ex- pression of CD4^＋ CD25^＋ CD127 /low Treg,CD3^＋ CD4^＋ T helper cells and CD19 ^＋ B cells in NS group were significantly decreased（P d0.05） ,while CD3^＋ CD8^＋ suppressor T cells were increased significantly （P〈0.05）. The expression of CD16^＋ CD56^＋ NK cells in children with NS was decreased, but there was no statistical significance when compared with control group（P〈0.05）. Conclusion The disorders of lymphocytes,especially the expression and functional changes of regulatory T cells may play a role in NS. The detection of regulatory T cells could contribute to the diagnosis and treatment of patients with NS.