摘要
目的:探讨转录激活因子3(STAT3)及X线修复交叉互补基因4(XRCC4)基因多态性与中国人群肝细胞癌(HCC)易感性的关系。方法采用病例对照研究,以确诊的200例乙型肝炎病毒(HBV)感染相关 HCC 为肝癌组,207例性别及年龄匹配的 HBsAg阳性患者为非肝癌组。采用PCR-限制性长度多态性技术检测STAT3 rs2292152和XRCC4 rs1805377多态性位点的基因型,Logistic回归分析比较不同基因型与 HCC易感风险的关系。结果(1)STAT3 rs2293152位点3种基因型(CC、CG和GG型)在肝癌组分布频率分别为17%(34/200)、49.5%(99/200)和33.5%(67/200),在非肝癌组中分别为18.4%(38/207)、56.5%(117/207)和25.1%(52/207),各基因型在两组之间的差异无统计学意义(P>0.05);以CC基因型作参照,携带rs2293152 GG型的个体 HCC患病风险差异无统计学意义(OR=1.440,95% CI=0.800~2.592,P=0.224)。(2)XRCC4 rs1805377位点3种基因型(AA、GA和GG 型)在肝癌组分布频率分别为61%(122/200)、30%(60/200)和9%(18/200),在非肝癌组中分别为59.9%(124/207)、31.9%(66/207)和8.2%(17/207),各基因型在两组之间的差异无统计学意义(P>0.05);以 AA 基因型作参照,携带rs1805377 GG型的个体 HCC 患病风险差异无统计学意义(OR=1.076,95% CI=0.530~2.185,P=0.839)。结论 STAT3 rs2292152和XRCC4 rs1805377多态性位点可能与中国人群HBV 相关HCC 易感性无密切关系。
Objective To investigate the association of genetic polymorphisms of STAT3 and XRCC4 with susceptibility to hep- atocellular carcinoma (HCC) in Chinese population. Methods In this case-control study,a total of 200 HCC patients infected with hepatitis B virus (HBV) and 207 age-and gender-matched non-HCC controls infected with HBV were recruited. Genetic polymor- phisms of STAT3 rs2293152 and XRCC4 rs1805377 were genotyped using polymerase chain reaction combined with restriction fragment length polymorphism method (PCR-RFLP). Logistic regression model was used to analyze the relationship between dif- ferent genotypes and their susceptibility to HCC. Results (1) The frequencies of CC,CG and GG genotype on STAT3 rs2293152 were 17% (34/200),49.5X (99/200)and 33.5% (67/200) respectively in the HCC group,and 18.4%(38/207),56.5%(117/ 207) and 25.1% (52/207) respectively in non-HCC group,and there was no statistical significance (P〈0.05). Compared with CC genotype,rs2293152 GG genotype carriers showed no significant different in the susceptibility to HCC (OR 1. 440,95%CI=0. 800-2. 592,P=0. 224). (2) The frequencies of AA,GA and GG genotypes on XRCC4 rs1805377 were 61% (122/200) ,30% (60/ 200) and 9% (18/200) respectively in the HCC group,while 59.9% (124/207) ,31.9% (66/207) and 8.2% (17/207) respectively in non-HCC group, and there was no statistical significance (P〉0.05). Compared with AA genotype, rs1805377 C,G genotype carriers showed no significant different in the susceptibility to HCC (OR=1. 076,95%CI=0. 530-2. 185 ,P=0. 839). Conclusion The polymor- phisms of STAT 3 rs2293152 and XRCC4 rs1805377 were not closely associated with susceptibility to HCC in Chinese population.
出处
《国际检验医学杂志》
CAS
2014年第7期850-852,共3页
International Journal of Laboratory Medicine
基金
南京市第一层次卫生人才项目(2011024)