Sysmex UF-500i全自动尿沉渣分析仪计数L型大肠埃希菌研究

Experimental research of counting Escherichia coli L-type bacterial by Sysmex UF-500i full-automatic urine sediment analyzer

目的分析Sysmex UF-500i全自动尿沉渣分析仪对大肠埃希菌L型细菌计数的特点。方法氨苄西林诱导大肠埃希菌成L型,观察细菌L型的菌落、形态、染色特点;配制一定菌液浓度,并用培养法验证配制菌液的实际浓度;用Sysmex UF-500i全自动尿沉渣分析仪对已知菌液浓度菌液进行细菌计数;比较大肠埃希菌L型理论浓度与Sysmex UF-500i全自动尿沉渣分析仪细菌计数结果的差异。结果氨苄西林极易诱导大肠埃希菌成为L型,细菌L型形态、染色与细菌型有极大不同;Sysmex UF-500i全自动尿液沉渣分析仪测定大肠埃希菌L型细菌计数结果远低于理论浓度。结论大肠埃希菌不稳定L型与相对稳定L型在Sysmex UF-500i全自动尿沉渣分析仪细菌计数结果,与细菌型计数不同;由于细菌的聚集性,所测定的细菌个数远低于实际细菌数;为了准确得出尿沉渣细菌计数结果,最好在临床用药前送检尿液标本。

Objective Analyze the characteristics of counting Escherichia coli L-type bacterial by UF-500i full-automatic urine sediment analyzer. Methods Induce Escherichia coli into Escherichia coli L-type with ampicillin,and observe the bacterial colony, form and dying characteristics. Prepare a certain concentration of bacteria fluid, and test the actual concentration by cultivation. Count bacteria with UF-500i full-automatic urine sediment analyzer. Compare the theoretical concentration of the Escherichia coli L-type with the result of bacteria count by LTF-500i full-automatic urinary sediment analyzer. Results F.scherichia coli could be eas- ily induced to L-type. Escherichia coli L-type and bacteria type were obviously different in form and staining. The bacterin count re- sult of Escherichia coli L-type,tested by Sysmex UF-500i full-automatic urinary sediment analyser was far lower than the theoreti- cal concentration. Conclusion The bacterin acount result of Escherchia coli L-type unstable and relatively stable,tested by Sysmex UF-500i full-automatic urinary sediment analyser is different from bacterin count. The number of the Escherichia coli L-type bacte- ria tested by Sysmex UF-500i full-automatic urinary sediment analyser is far less to actual number of bacteria, due to bacterium ag- gregation. In order to draw an accurate urine bacterial count results, the urine specimens been transported and tested before clinical treatment is best.