七氟醚后处理对大鼠脑缺血-再灌注损伤的抗炎作用

Anti-inflammatory effect of sevoflurane postconditioning on cerebral ischemia-reperfusion injury in rats

目的观察七氟醚后处理对大鼠脑缺血-再灌注(IR)炎症反应的作用。方法 40只Wistar大鼠被随机分为五组:S组大鼠分离血管但不阻断大脑中动脉,术后90min给予1.0MAC的七氟醚30min。IR组阻断大脑中动脉90min,在再灌注初期给予氧气30min。PS1、PS2、PS3组均阻断大脑中动脉90min,在再灌注初期分别给予0.5、1.0、1.5MAC的七氟醚30min。再灌注24h时采集股动脉血样,测定血清TNF-α、IL-10、IL-1β浓度。结果与S组比较,IR组大鼠的血清TNF-α和IL-1β浓度明显升高,而IL-10浓度明显降低(P<0.05)。与IR组比较,PS1、PS2和PS3组血清TNF-α和IL-1β浓度明显降低,IL-10浓度明显升高(P<0.05),并且随着七氟醚吸入浓度的增加,其作用不同程度地增强(P<0.05)。结论七氟醚后处理对于缺血-再灌注大鼠可实现脑保护作用,并缓解血清炎症因子的变化。

Objective To observe the effects of different concentrations of sevoflurane postcon- ditioning on inflammatory reactions in rats with cerebral ischemia-reperfusion injury following middle cerebral artery occlusion （MCAO）. Methods Forty Wistar rats were randomly assigned to groups I V. Rats in the sham group （group S） were subjected to the vessels isolation without occlusion of the middle cerebral artery and exposed to 1.0 MAC sevoflurane at 90 min after surgery for 30 rain. Rats in ischemia-reperfusion group （group IR） were subjected to MCAO for 90 rain and then treated with 02 for 30 min at the beginning of reperfusion. Sevoflurane postconditing groups 1, 2, and 3 （groups PS1, PS2, and PS3） were subjected to MCAO for 90 rain, and then exposed to 0.5, 1.0, or 1.5 MAC sevoflurane for 30 min at the beginning of reperfusion, respectively. Blood samples were collected from femoral artery at 24 h after reperfusion, and serum TNF-a, IL-10, IL-1β were measured. Results Compared with group S, the serum concentrations of TNF-a and IL-1β in group IR were increased, whereas the concentration of IL-10 was decreased significantly （P〈0.05）. Compared with group IR, serum concentration of TNF-a, IL-1β were decreased and serum concentration of IL-10 was increased in groups PS1, PS2, PS3 （P〈0.05）in a dose-dependent manner. Conclusion Sevoflurane postconditioning not only exerts neuroprotective effect on inschemia-reperfused rats but also ameliorates inflammatory reactions.