深绿木霉发酵液对黑麦草促生作用及生理生化特性的影响

Effects of fermentation medium of Trichoderma aureoviride on growth and physiological characteristics of ryegrass

通过种子萌发和幼苗盆栽试验研究了不同稀释倍数深绿木霉发酵液对黑麦草促生作用及生理生化特性的影响。室内试验结果表明,不同稀释倍数深绿木霉发酵液能够显著提高黑麦草种子的发芽率、发芽指数和活力指数,尤其是100倍稀释液对黑麦草种子发芽率、发芽指数和活力指数的影响较为明显,分别为96.55%、37.41和7.83。盆栽试验结果表明,不同稀释倍数深绿木霉发酵液对黑麦草幼苗的生长具有明显的促生作用,以及能够显著地提高幼苗叶绿素、可溶性蛋白含量和与抗性相关酶的活性,尤其是100倍稀释液处理的黑麦草幼苗根系长度、株高、鲜重、干重和根冠比的相对增长率分别为16.95%、13.33%、40.57%、73.68%和36.36%;叶绿素和可溶性蛋白含量的相对增长率分别为84.09%和75.50%;多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶活性均显著高于对照,且多酚氧化酶和苯丙氨酸解氨酶活性在处理后第四天达到最大值,过氧化物酶活性在处理后第三天达到最大值。因此,深绿木霉发酵液对黑麦草的生长具有显著的促生作用,且能够显著提高与抗性相关酶的活性。

Effects of different dilutions of the fermentation medium of Trichoderma aureoviride on growth and physio-logical characteristics of ryegrass were studied by seed germination and seedling pot experiment in vitro and pot experi -ments .Results showed that different dilutions of the fermentation medium of T .aureoviride can significantly increase the rates of seed germination ,germination index and vigor index ,especially for the dilutions of 100 times by which seed ger-mination rates ,germination index and vigor index were increased by 96 .55% ,37 .41 and 7 .83 ,respectively .Pot exper-iment results showed that different dilutions of the fermentation medium of T . aureoviride had obvious effects on the growth of ryegrass seedlings ,and can significantly increase the content of chlorophyll ,protein ,and defensive enzymes . Especially the dilutions of 100 times can increase ryegrass root length ,plant height ,fresh weight and dry weight by 16 .95% 、13 .33% 、40 .57% 、73 .68% ,and 36 .36% ,respectively .The content of chlorophyll and soluble protein were increased by 84 .09% and 75 .50% .Polyphenol oxidase ,peroxidase and phenylalanine enzyme activity were significantly higher than control after treatment with the dilutions of 100 times of T . aureoviride .Maximum activities of POD ,PPO and PAL were observed on 3 and 4 days after inoculation .Therefore ,the results suggested that the fermentation medium＆amp;nbsp;of T . aureoviride has substantial effects on growth and physiological characteristics of ryegrass .