卵母细胞体外成熟培养液的改良及研究

The improvement and research of the cultured medium in in-vitro maturation

目的探讨卵母细胞体外成熟培养液的改良及研究。方法选取昆明小鼠50只作为研究对象，根据培养液的不同将50只小鼠分为A组和B组，每组各25只。A组采用低糖细胞培养基（DMEM）培养，B组采用含15％～20％胎牛血清的人类输卵管液培养，两组在相同条件下进行培养，比较两组的体外受精率、正常受精率、卵裂率、优质胚胎率、可移植胚胎率、妊娠率及活产率。结果A组小鼠的体外受精率（71．4％）、正常受精率（65．7％）及卵裂率（89．1％）与B组（67．6％、68．1％、85．1％）比较，差异无统计学意义（P〉0．05）；A组小鼠的优质胚胎率（31．7％）和可移植胚胎率（56．1％）均优于B组（25．0％、42．5％），差异有统计学意义（X2=8．26、10．17，P〈0．05）。A组小鼠的妊娠率（23.3％）低于B组（32．6％），差异有统计学意义（X2=7．58，P〈0．05）；但A组小鼠的活产率（17．8％）稍低于B组（18．9％）比较，差异无统计学意义（P〉O．05）。结论低糖DMEM培养液具有较好的应用效果，但仍需进一步的研奔．

Objective To explore the improvement and research of the cultured medium in in--vitro maturation. Meth- ods 50 Kunming mice were selected as the research objects, they were divided into group A and group B according to the different cultured medium, each group of 25 mice. Group A was given low sugar dulbecco＇s modified eagle medium （DMEM） culture, group B was given human tube fluid containing 15%-20% fetal bovine serum, two groups under the same conditions, the in-vitro fertilization rate, normal fertilization rate, cleavage rate, high quality embryo rate, portable embryo rate and pregnancy rate and live birth rates in two groups were compared. Results The in-vitro fertilization rate （71.4%）, normal fertilization rate （65.7%） and cleavage rate （89.1%） in group A of mice compared with group B （67.6%, 68.1%, 85.1%）, there were no statistically significant differences （P 〉 0.05）, the high quality of mice rates of embryo （31.7%） and portable embryos （56.1%） in group A were better than those of group B, the differences were statistically significant （X2=8.26, 10.17, P 〈 0.05）. The pregnancy rate in group A （23.3%） was less than that of group B （32.6%）, the difference was statistically significant （X2=7.58, P 〈 0.05）, but the live birth rate of mice in group A （17.8%） was little lower than that of group B （18.9%）, there was no statistically significant difference （P 〉 0.05）. ConcLusion The low sugar DMEM culture has good using effect, but still needs further research.