电脉冲化疗对人前列腺癌细胞系PC-3M的体外作用

Pingyangmycin(bleomycin A5)-mediated electrochemotherapy on human prostate cancer cell line PC-3M in vitro

目的 探讨电脉冲化疗对人前列腺癌细胞系PC 3M的体外作用。 方法 选用平阳霉素作为抗癌药物与电脉冲相结合 ,测定电脉冲化疗对PC 3M细胞系的细胞毒作用。HE染色法观察实验后形态学改变。 结果 单用平阳霉素 ,低浓度无效 ,高浓度有效 ,最大无细胞毒作用浓度为0 .1μg/ml;单用电脉冲 ,最大无细胞毒作用场强为 12 5V/mm ;平阳霉素与电脉冲相结合的电脉冲化疗最大无细胞毒作用浓度仅为单用平阳霉素的 1/ 10 0 ;0 .1μg/ml的平阳霉素与 12 5V/mm的场强相结合 ,细胞生存分数为 5 2 .6 % ,组织形态学改变明显。 结论 平阳霉素可显著被电脉冲增效 ,适用于电脉冲化疗 ;平阳霉素介导的电脉冲化疗对前列腺癌细胞生长有较强的抑制作用。

Objective To investigate the efficacy of electrochemotherapy with pingyangmycin (bleomycin A5) for the treatment of human prostate cell line PC 3M in vitro. Methods The prostate cancer cells were treated by pingyangmycin alone, electric pulse alone and by electrochemotherapy, i.e. electric pulses were delivered after pingyangmycin was given. Cytotoxicity was examined by MTT assay and histological examination. Results The maximum nontoxic dose for pingyangmycin was 0.1 μg/ml; The maximum voltage not showing cytotoxicity was 125 V/mm.The 50% inhibitory concentration was 100 times reduced in electrochemotherapy than pingyangmycin alone. Conclusions The cytotoxicity of pingyangmycin can be enhanced by electric impulses. Pingyangmycin may be a novel drug for electrochemotherapy.