云南切梢小蠹微卫星的高通量发掘

High- throughput discovery microsatellites in Tomicus yunnanensis( Coleoptera: Scolytinae)

基于构建获得的云南切梢小蠹转录组数据库,利用MISA(MicroSatellite)软件对其微卫星进行了高通量发掘。在32595681 bp的转录组序列中,共发掘获得1098个微卫星序列。在这些微卫星序列中,单核苷酸和三核苷酸微卫星重复单元最为丰富,分别为420和482个;六核苷酸的微卫星重复单元的丰度最小,仅有3个。就重复序列而言,以A/T单碱基重复序列最多,有367个;其次是AAT/ATT和ATC/ATG三碱基序列,有83个;最少的是CG/CG二碱基序列,有2个。研究结果为开发高多态性微卫星引物来进行云南切梢小蠹种群遗传结构、种群遗传多样性、功能基因组学等研究奠定了基础。

Based on the constructed transcriptome database of Tomicus yunnanensis （Coleoptera： Scolytinae）, microsatellites of this forestry pest have been high -throughput discovered. In total, 1098 microsatellites were identified from the 32595681 bp transcriptome sequences. Among these microsatellites, mono - and trinucieotide were the most abundant repeats, of which were 420 and 482, respectively. Hexanucieotide repeats were the least abundant, and only 3 of such type were observed. With respect to the repeat type, A/T repeats （367） were the most abundant type, followed by AAT/ATT and ATC/ATG repeats （83）. CG/CG repeats （2） were the least type. The results provide a platform for developing high polymorphic microsatellites to investigate the population genetic structure, genetic diversity and functional genomics of T. yunnanensis.