黑龙江省主栽水稻品种抗稻瘟病基因Pi-2(t)的SSR检测分析

Identification of Rice Blast Resistance Gene Pi-2(t) in Rice Varieties Commercially Grown in Heilongjiang Province with SSR Marker

为合理搭配种植水稻品种,利用与抗稻瘟病基因Pi-2(t)紧密连锁的SSR标记AP22和SRM24检测49份黑龙江省主栽水稻品种(系)抗瘟基因Pi-2(t)的分布情况。结果表明:用2个与抗稻瘟病基因Pi-2(t)紧密连锁的SSR标记同时对抗瘟基因Pi-2(t)的检测是非常有效的方法,龙粳8号、绥粳4号、龙D99-709和垦676共4个水稻品种(系)检测出含有Pi-2(t)抗稻瘟病基因,并了解了该基因在黑龙江省水稻品种资源中的分布情况。

In order to reasonably collocat planting rice varieties,distribution of the blast resistance gene Pi-2（t） in 49 rice varieties commercially grown in Heilongjiang province was identified by the simple sequence repeat （SSR）marker AP22 and SRM24,which was tightly linked to blast resistance gene Pi-2 （t） in rice. The results showed the indentificaition was very effective; Varieties of Longjing 8, Suijing 4, Long D99-709 and Ken676 were identified to have the Pi-2（t） gene;Distribution of the Pi-2（t） gene in rice resources was discussed.